Corrigendum
for Houghton1 et al., Hum. Reprod. 17 (4) 999-1005.
Human Reproduction, Vol. 18, No. 8, 1756-1757,
August 2003
© 2003 European Society of Human Reproduction and Embryology
Non-invasive amino acid turnover predicts human embryo developmental capacity
F.D. Houghton,
J.A. Hawkhead,
P.G. Humpherson,
J.E. Hogg,
A.H. Balen,
A.J. Rutherford and
H.J. Leese
Non-invasive amino acid turnover predicts human embryo developmental capacity
By F.D.Houghton, J.A.Hawkhead, P.G.Humpherson, J.E.Hogg, A.H.Balen, A.J.Rutherford and H.J.Leese
Hum. Reprod., 17, 999–1005, 2002
Errors have been found in the above paper and the corrections are given below.
Due to a calculation error, the amino acid values presented for 17 of the 18 amino acids in the above paper were 12.5 times too high. This applies to all the amino acids except arginine (Arg) which was 1.25 times too high. The error is on the y axis of the Figures and does not affect the conclusions in any way. As regards statistical significance, there are no changes to Figures 1, 3 and 4, but some minor alterations are necessary in Figures 2 and 5. In Figure 2, the amino acid turnover for the arresting embryos is now significant at **P < 0.01 instead of ***P < 0.001. In Figure 5, total amino acid depletion has become more significant in developing embryos; between day 2-3 and c8-cell to morula, P = 0.01; between day 2-3 and the morula and blastocyst stage, P = 0.001; between the c8-cell to morula and the morula to blastocyst, P = 0.05. There is now no significant difference in amino acid depletion between the developing and arresting embryos at the c8-cell to morula stage (Figure 5).
The revised figures are given below.
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Figure 1. Amino acid depletion (negative values) and appearance (positive values) ± SEM by individual, spare human preimplantation embryos which subsequently formed blastocysts (n = 14), or which arrested in culture prior to blastocyst formation (n = 28), measured non-invasively from day 2–3 of development. *P < 0.05; **P < 0.01; ***P < 0.001 significance from zero. Bars with the same superscript are significantly different: a, P = 0.000; b, P = 0.023; c, P = 0.026; d, P = 0.033; e, P = 0.044.
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Figure 2. Amino acid turnover for those embryos which subsequently developed to the blastocyst stage compared with those which arrested prior to blastocyst formation. Values are the sum of amino acids that significantly appeared/disappeared from the medium ± SEM. **P < 0.01; ***P < 0.001 significantly different from embryos that developed to the blastocyst stage.
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Figure 3. Amino acid depletion/appearance ± SEM by individual human embryos over the compacting 8-cell to morula transition which subsequently formed blastocysts (n = 23), or which arrested in culture prior to cavitation (n = 32). *P < 0.05; **P < 0.01; ***P < 0.001 significance from zero. Bars with the same superscripts are significantly different: a, P = 0.015; b, P = 0.019; c, P = 0.037. c8-cell = compacting 8-cell.
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Figure 4. Amino acid depletion/appearance ± SEM by individual human embryos over the morula to blastocyst transition (n = 42). **P < 0.01; ***P < 0.001 significance from zero.
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Figure 5. Total depletion of amino acids from the medium ± SEM by developing and arresting human embryos from day 2 through to the blastocyst stage. ***P < 0.001 significantly different from embryos that developed to the blastocyst stage. Bars with the same superscript are significantly different; a, P = 0.05; b, P = 0.01; c, P = 0.001.
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