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Human Reproduction, Vol. 11, No. 6, pp. 1306-1308, 1996
© 1996 European Society of Human Reproduction and Embryology

case-report

Pregnancy resulting from intracytoplasmic injection of cryopreserved spermatozoa recovered from testicular biopsy

Barbara T. Podsiadly1, Robert J. Woolcott, James D. Stanger and Karen Stevenson

Lingard Fertility Centre 23 Merewether Street, Merewether, NSW 2291, Australia

Correspondence: 1To whom correspondence should be addressed

We report a clinical pregnancy occurring in a 31 year old patient following intracytoplasmic sperm injection (ICSI) of cryopreserved spermatozoa obtained from a testicular biopsy. This was the couple‘s second attempt at an ovarian stimulated cycle resulting in the collection of 17 metaphase II ova which were all injected with progressively motile spermatozoa. A fertilization rate of 58% and a cleavage rate of 90% were achieved. This report is our first case of ICSI using cryopreserved testicular spermatozoa which resulted in normal fertilization, embryo development and an on-going singleton pregnancy.

Key words: cryopreserved testicular sperm/ICSI/male infertility


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