Human Reproduction, Vol. 11, No. 6, pp. 1309-1313, 1996
© 1996 European Society of Human Reproduction and Embryology
research-article |
Pregnancies after intracytoplasmic sperm injection with cryopreserved testicular spermatozoa
1Instituto Valenciano de Infertilidad Guardia Civil 23, E-46020 Valencia 2Division of Urology, Department of Surgery, Valencia University School of Medicine Av. Blasco Iánez 17, E-46010 Valencia, Spain
Correspondence: 3To whom correspondence should be addressed
In 25 patients (14 suffering from obstructive azoospermia, six from non-obstructive azoospermia, three from astheno-azoospermia and two from absence of ejaculation) spermatozoa were extracted from testicular biopsies. Intracytoplasmic sperm injection (ICSI) with fresh testicular spermatozoa was performed in 18 cases; spermatozoa in excess were cryopreserved in pills. No pregnancies were achieved. In the remaining seven patients, testicular spermatozoa were retrieved and cryopreserved during a diagnostic testicular biopsy. After thawing, sperm motility was assessed in 17 cases (68%), and 18 ICSI with cryopreserved testicular spermatozoa were performed. The mean two-pronuclear (2PN) fertilization rate was 59%, the mean cleavage rate was 92%, and six clinical pregnancies were achieved, all of them still ongoing (pregnancy rate 33%). A comparison of the results of ICSI carried out with fresh or cryopreserved testicular spermatozoa showed that the mean 2PN fertilization rates per cycle (53 compared with 55%), mean cleavage rates per cycle (99 compared with 96%) and embryo quality were not significantly different In conclusion, cryopreservation of testicular spermatozoa is feasible, even in patients with non-obstructive azoospermia, and the results of ICSI with frozen-thawed testicular spermatozoa are similar to those obtained using fresh testicular spermatozoa. Cryopreservation of testicular spermatozoa may avoid repetition of testicular biopsies to retrieve spermatozoa for successive ICSI cycles in patients in whom the only source of motile spermatozoa is the testicle.
Key words: intracytoplasmic sperm injection/male infertility/pregnancy/sperm cryopreservation/testicular biopsy
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