Human Reproduction, Vol. 11, No. 6, pp. 1318-1323, 1996
© 1996 European Society of Human Reproduction and Embryology
research-article |
The production of tumour necrosis factor a (TNF-
) by human endometrial cells in culture
1Division of Biomedical Sciences/Health Research Institute Sheffield Hallam University Pond Street, Sheffield S1 1WB 2Biomedical Research Unit, Jessop Hospital for Women Leavygreave Road, Sheffield, S3 7RE, UK
Correspondence: 3To whom correspondence should be addressed
The production of tumour necrosis factor a (TNF-
) by cultured human endometrial epithelial and stromal cells prepared from endometrium obtained at different stages in the menstrual cycle has been investigated. TNF- was not detectable in the supernatants of stromal cell cultures prepared from endometrial tissue obtained at any time in the menstrual cycle. TNF- production by endometrial epithelial cells in culture varied depending on the time in the cycle at which the endometrial tissue was taken. Cells prepared from tissue obtained during the late proliferative phase of the cycle produced more TNF- than those prepared from tissue obtained at other times in the cycle. In addition, a small increase in TNF- production was seen by cells prepared from tissue obtained during the mid-secretory phase of the cycle. Interieukin 1 (IL-1) (1.4–140 pmol/1) caused a dose-dependent increase in TNF- production by cells prepared from both proliferative and secretory endometrium. Maximum LL-1-stimulated increases in TNF- production were similar in cells from both proUferative and secretory endometrium and typically reached from four to 10 times basal values. High doses of progesterone, either alone or in the presence of oestradiol, also affected TNF-a production by epithelial cells. TNF- production by cells prepared from proliferative endometrium was increased by progesterone. In contrast, TNF- production by cells prepared from secretory endometrium was decreased in the presence of progesterone. The effects of steroids on TNF- production were less marked than that of IL-1, with values increasing or decreasing to a maximum of three times the basal value. Placental protein 14 (PP14) (0.18 and 1.8 nmol/1) also increased TNF- production by cells prepared from proliferative tissue, but had no effect on its production by cells prepared from secretory endometrium. PP14-stimulated TNF- levels typically only reached a maximum of two times basal values.
Key words:
human endometrium/interleukin 1/placental protein 14/steroids/tumour necrosis factor
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  M. Wahab, A.H. Taylor, J.H. Pringle, J. Thompson, and F. Al-Azzawi Trimegestone differentially modulates the expression of matrix metalloproteinases in the endometrial stromal cell Mol. Hum. Reprod., March 1, 2006; 12(3): 157 - 167. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. D. Mueller, J.-L. Vigne, M. Streich, M. K. Tee, L. Raio, E. Dreher, N. A. Bersinger, and R. N. Taylor 2,3,7,8-Tetrachlorodibenzo-p-Dioxin Increases Glycodelin Gene and Protein Expression in Human Endometrium J. Clin. Endocrinol. Metab., August 1, 2005; 90(8): 4809 - 4815. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Bouman, M. J. Heineman, and M. M. Faas Sex hormones and the immune response in humans Hum. Reprod. Update, July 1, 2005; 11(4): 411 - 423. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Lalitkumar, J Sengupta, and D Ghosh Endometrial tumor necrosis factor {alpha} (TNF{alpha}) is a likely mediator of early luteal phase mifepristone-mediated negative effector action on the preimplantation embryo Reproduction, March 1, 2005; 129(3): 323 - 335. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Thathiah, M. Brayman, N. Dharmaraj, J. J. Julian, E. L. Lagow, and D. D. Carson Tumor Necrosis Factor {alpha} Stimulates MUC1 Synthesis and Ectodomain Release in a Human Uterine Epithelial Cell Line Endocrinology, September 1, 2004; 145(9): 4192 - 4203. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. R. Gonzalez, K. Leary, J. C. Petrozza, and P. C. Leavis Leptin regulation of the interleukin-1 system in human endometrial cells Mol. Hum. Reprod., March 1, 2003; 9(3): 151 - 158. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Wieser, G. Fabjani, C. Tempfer, C. Schneeberger, R. Zeillinger, J. C. Huber, and R. Wenzl Tumor Necrosis Factor-{alpha} Promotor Polymorphisms and Endometriosis Reproductive Sciences, September 1, 2002; 9(5): 313 - 318. [Abstract] [PDF]
|
|
|
|
|
|
B.A. Cork, E.M. Tuckerman, T.C. Li, and S.M. Laird Expression of interleukin (IL)-11 receptor by the human endometrium in vivo and effects of IL-11, IL-6 and LIF on the production of MMP and cytokines by human endometrial cells in vitro Mol. Hum. Reprod., September 1, 2002; 8(9): 841 - 848. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Sugino, A. Karube-Harada, A. Sakata, S. Takiguchi, and H. Kato Nuclear Factor-{kappa}B Is Required for Tumor Necrosis Factor-{alpha}-Induced Manganese Superoxide Dismutase Expression in Human Endometrial Stromal Cells J. Clin. Endocrinol. Metab., August 1, 2002; 87(8): 3845 - 3850. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y.C. Cheong, J.B. Shelton, S.M. Laird, M. Richmond, G. Kudesia, T.C. Li, and W.L. Ledger IL-1, IL-6 and TNF-{alpha} concentrations in the peritoneal fluid of women with pelvic adhesions Hum. Reprod., January 1, 2002; 17(1): 69 - 75. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Karube-Harada, N. Sugino, S. Kashida, S. Takiguchi, H. Takayama, Y. Yamagata, Y. Nakamura, and H. Kato Induction of manganese superoxide dismutase by tumour necrosis factor-{alpha} in human endometrial stromal cells Mol. Hum. Reprod., November 1, 2001; 7(11): 1065 - 1072. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Hague, L. Zhang, M. K. Oehler, S. Manek, I. Z. MacKenzie, R. Bicknell, and M. C. P. Rees Expression of the Hypoxically Regulated Angiogenic Factor Adrenomedullin Correlates with Uterine Leiomyoma Vascular Density Clin. Cancer Res., July 1, 2000; 6(7): 2808 - 2814. [Abstract] [Full Text]
|
|
|
|
|
|
S.M. Laird, E.M. Tuckerman, B.A. Cork, and T.C. Li Expression of nuclear factor {kappa} B in human endometrium; role in the control of interleukin 6 and leukaemia inhibitory factor production Mol. Hum. Reprod., January 1, 2000; 6(1): 34 - 40. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Ghosh, P.G.L. Lalitkumar, V. J. Wong, A.G. Hendrickx, and J. Sengupta Preimplantation embryo morphology following early luteal phase anti-nidatory treatment with mifepristone (RU486) in the rhesus monkey Hum. Reprod., January 1, 2000; 15(1): 180 - 188. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C.F. Singer, E. Marbaix, P. Lemoine, Y. Eeckhout, and P.J. Courtoy Interleukin-1 receptor-ligand interactions modulate interstitial collagenase-1 production by human endometrial fibroblasts Mol. Hum. Reprod., March 1, 1999; 5(3): 240 - 245. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. von Wolff, I. Classen-Linke, D. Heid, C.A. Krusche, K. Beier-Hellwig, C. Karl, and H.M. Beier Tumour necrosis factor-{alpha} (TNF-{alpha}) in human endometrium and uterine secretion: an evaluation by immunohistochemistry, ELISA and semiquantitative RT–PCR Mol. Hum. Reprod., February 1, 1999; 5(2): 146 - 152. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. N. Taylor, J.-F. Savouret, C. Vaisse, J.-L. Vigne, I. Ryan, D. Hornung, M. Seppälä, and E. Milgrom Promegestone (R5020) and Mifepristone (RU486) Both Function as Progestational Agonists of Human Glycodelin Gene Expression in Isolated Human Epithelial Cells J. Clin. Endocrinol. Metab., November 1, 1998; 83(11): 4006 - 4012. [Abstract] [Full Text]
|
|