Human Reproduction, Vol 12, 815-822, Copyright © 1997 by Oxford University Press
B Viville, DS Charnock-Jones, AM Sharkey, B Wetzka and SK Smith
The two forms of the progesterone receptor, PR-A and PR-B, are
independently regulated at the transcriptional level, and show distinct
responses to progesterone antagonists. We were interested in possible
differences in the PR-A to PR-B ratio between uterine myometrium and
leiomyomata (fibroid), that might influence the response of fibroids to
progesterone agonists and antagonists, and thus have consequences for the
treatment of this condition. Fibroid and adjacent normal myometrium were
obtained from 11 women undergoing hysterectomy. Immunohistochemistry using
a monoclonal antibody which recognizes both PR-A and PR-B showed
exclusively nuclear staining, and this was stronger in the leiomyomata than
in adjacent myometrium. An antibody specific for PR-B gave fainter staining
of both tissues. Western blotting confirmed a higher concentration of PR in
leiomyomata than myometrium in eight out of 11 cases. In all cases both
forms were present, with a consistent dominance of PR-A over PR-B. However
an RNase protection assay showed that there was no difference between the
concentrations of mRNA encoding PR-A and PR-B, or between the mRNA
concentrations in leiomyomata and normal myometrium. We conclude that the
observed differences between the levels of immunoreactive PR in leiomyomata
and myometrium may result from post-translational control, and support the
use of progesterone antagonists in the treatment of leiomyomata.
ARTICLES
Distribution of the A and B forms of the progesterone receptor messenger ribonucleic acid and protein in uterine leiomyomata and adjacent myometrium
Department of Obstetrics and Gynaecology, University of Cambridge, Rosie Maternity Hospital, UK.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  D.-c. Kim, S. Ramachandran, S.-h. Baek, S.-H. Kwon, K.-Y. Kwon, S.-D. Cha, I. Bae, and C.-H. Cho Induction of Growth Inhibition and Apoptosis in Human Uterine Leiomyoma Cells by Isoliquiritigenin Reproductive Sciences, July 1, 2008; 15(6): 552 - 558. [Abstract] [PDF]
|
|
|
|
|
|
N. Ohara, A. Morikawa, Wei Chen, Jiayin Wang, D. A. DeManno, K. Chwalisz, and T. Maruo Comparative Effects of SPRM Asoprisnil (J867) on Proliferation, Apoptosis, and the Expression of Growth Factors in Cultured Uterine Leiomyoma Cells and Normal Myometrial Cells Reproductive Sciences, December 1, 2007; 14(8_suppl): 20 - 27. [Abstract] [PDF]
|
|
|
|
 |
|
 M. H. Hassan, S. A. Salama, H. M. M. Arafa, F. M. A. Hamada, and A. Al-Hendy Adenovirus-Mediated Delivery of a Dominant-Negative Estrogen Receptor Gene in Uterine Leiomyoma Cells Abrogates Estrogen- and Progesterone-Regulated Gene Expression J. Clin. Endocrinol. Metab., October 1, 2007; 92(10): 3949 - 3957. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Q. Xu, N. Ohara, W. Chen, J. Liu, H. Sasaki, A. Morikawa, R. Sitruk-Ware, E. D.B. Johansson, and T. Maruo Progesterone receptor modulator CDB-2914 down-regulates vascular endothelial growth factor, adrenomedullin and their receptors and modulates progesterone receptor content in cultured human uterine leiomyoma cells Hum. Reprod., September 1, 2006; 21(9): 2408 - 2416. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. Chen, N. Ohara, J. Wang, Q. Xu, J. Liu, A. Morikawa, H. Sasaki, S. Yoshida, D. A. Demanno, K. Chwalisz, et al. A Novel Selective Progesterone Receptor Modulator Asoprisnil (J867) Inhibits Proliferation and Induces Apoptosis in Cultured Human Uterine Leiomyoma Cells in the Absence of Comparable Effects on Myometrial Cells J. Clin. Endocrinol. Metab., April 1, 2006; 91(4): 1296 - 1304. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Zaitseva, B. J. Vollenhoven, and P. A.W. Rogers In vitro culture significantly alters gene expression profiles and reduces differences between myometrial and fibroid smooth muscle cells Mol. Hum. Reprod., March 1, 2006; 12(3): 187 - 207. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C.D. Swartz, C.A. Afshari, L. Yu, K.E. Hall, and D. Dixon Estrogen-induced changes in IGF-I, Myb family and MAP kinase pathway genes in human uterine leiomyoma and normal uterine smooth muscle cell lines Mol. Hum. Reprod., June 1, 2005; 11(6): 441 - 450. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Maruo, N. Ohara, J. Wang, and H. Matsuo Sex steroidal regulation of uterine leiomyoma growth and apoptosis Hum. Reprod. Update, May 1, 2004; 10(3): 207 - 220. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Inoue, J.-I. Akahira, T. Suzuki, A. D. Darnel, C. Kaneko, K. Takahashi, M. Hatori, R. Shirane, T. Kumabe, Y. Kurokawa, et al. Progesterone Production and Actions in the Human Central Nervous System and Neurogenic Tumors J. Clin. Endocrinol. Metab., November 1, 2002; 87(11): 5325 - 5331. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Leyendecker, M. Herbertz, G. Kunz, and G. Mall Endometriosis results from the dislocation of basal endometrium Hum. Reprod., October 1, 2002; 17(10): 2725 - 2736. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Danilovich, I. Roy, and M. R. Sairam Emergence of Uterine Pathology during Accelerated Biological Aging in FSH Receptor-Haploinsufficient Mice Endocrinology, September 1, 2002; 143(9): 3618 - 3627. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Condon, S. Yin, B. Mayhew, R. A. Word, W.E. Wright, J.W. Shay, and W. E. Rainey Telomerase Immortalization of Human Myometrial Cells Biol Reprod, August 1, 2002; 67(2): 506 - 514. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. L. Walker Role of Hormonal and Reproductive Factors in the Etiology and Treatment of Uterine Leiomyoma Recent Prog. Horm. Res., January 1, 2002; 57(1): 277 - 294. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. R. ATTIA, K. ZEITOUN, D. EDWARDS, A. JOHNS, B. R. CARR, and S. E. BULUN Progesterone Receptor Isoform A But Not B Is Expressed in Endometriosis J. Clin. Endocrinol. Metab., August 1, 2000; 85(8): 2897 - 2902. [Abstract] [Full Text]
|
|
|
|
 |
|
 S. Hague, L. Zhang, M. K. Oehler, S. Manek, I. Z. MacKenzie, R. Bicknell, and M. C. P. Rees Expression of the Hypoxically Regulated Angiogenic Factor Adrenomedullin Correlates with Uterine Leiomyoma Vascular Density Clin. Cancer Res., July 1, 2000; 6(7): 2808 - 2814. [Abstract] [Full Text]
|
|
|
|
 |
|
 L. C. Hodges, J. S. Bergerson, D. S. Hunter, and C. L. Walker Estrogenic Effects of Organochlorine Pesticides on Uterine Leiomyoma Cells in Vitro Toxicol. Sci., April 1, 2000; 54(2): 355 - 364. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Sahlin, H. Wang, B. Lindblom, H. Eriksson, A. Holmgren, and A. Blanck Thioredoxin expression in human myometrium and fibroids Mol. Hum. Reprod., January 1, 2000; 6(1): 60 - 67. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M.A. Habiba, S.C. Bell, and F. Al-Azzawi The effect of hormone replacement therapy on the immunoreactive concentrations in the endometrium of oestrogen and progesterone receptor, heat shock protein 27, and human {beta}-lactoglobulin Hum. Reprod., January 1, 2000; 15(1): 36 - 42. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Nisolle, S. Gillerot, F. Casanas-Roux, J. Squifflet, M. Berliere, and J. Donnez Immunohistochemical study of the proliferation index, oestrogen receptors and progesterone receptors A and B in leiomyomata and normal myometrium during the menstrual cycle and under gonadotrophin-releasing hormone agonist therapy Hum. Reprod., November 1, 1999; 14(11): 2844 - 2850. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. G. Shanker and A. Jagannadha Rao Progesterone receptor expression in the human placenta Mol. Hum. Reprod., May 1, 1999; 5(5): 481 - 486. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Noe, G. Kunz, M. Herbertz, G. Mall, and G. Leyendecker The cyclic pattern of the immunocytochemical expression of oestrogen and progesterone receptors in human myometrial and endometrial layers: characterization of the endometrial–subendometrial unit Hum. Reprod., January 1, 1999; 14(1): 190 - 197. [Abstract] [Full Text] [PDF]
|
|