Human Reproduction, Vol 12, 1056-1058, Copyright © 1997 by Oxford University Press
WR Edirisinghe, SM Junk, PL Matson and JL Yovich
This case report describes the birth of a baby following the transfer of
cryopreserved embryos generated from intracytoplasmic sperm injection
(ICSI) carried out on the second day after oocyte pick-up of
in-vitro-matured metaphase I and germinal vesicle stage oocytes. The couple
had a history of three failed intrauterine insemination attempts and
reduced fertilization rates in two previous in-vitro fertilization (IVF)
cycles. In the IVF-ICSI treatment cycle, 6/11 mature oocytes became
fertilized following ICSI on the first day. However, the patient failed to
conceive following the transfer of three embryos. Five oocytes were
immature (two at metaphase I stage and three with a germinal vesicle) and
these were cultured overnight. All had extruded a polar body by the
following day and ICSI was therefore performed; four oocytes became
fertilized, and were cryopreserved at the pronulear stage in propanediol.
In the next treatment cycle, transfer of frozen embryos was planned. The
pronuclear zygotes were thawed and cultured for 24 h prior to the transfer
of two embryos in a cycle stimulated with low doses of follicle stimulating
hormone. This resulted in a pregnancy and the delivery of a healthy baby
boy. In-vitro maturation of metaphase I and germinal vesicle oocytes which
are routinely collected in IVF-ICSI cycles, followed by second day ICSI
fertilization, may provide a valuable source of embryos for infertile
couples.
ARTICLES
Birth from cryopreserved embryos following in-vitro maturation of oocytes and intracytoplasmic sperm injection
PIVET Medical Centre, Leederville, Perth, Western Australia.
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