Human Reproduction, Vol 12, 1271-1274, Copyright © 1997 by Oxford University Press
M Alsalili, S Thornton and S Fleming
Propofol is a common anaesthetic agent used for oocyte retrieval procedures
during in-vitro fertilization (IVF). The effect of Propofol in vitro on
mouse oocyte maturation, fertilization and embryo cleavage was studied. In
this study, 551 cumulus-free and 222 cumulus-enclosed oocytes from mice
stimulated with pregnant mare's serum gonadotrophin (PMSG) were incubated
for 30 min in medium containing 0, 100, 1000 or 10,000 ng/ml of Propofol
prior to in-vitro maturation. Also, 325 cumulus-enclosed oocytes from mice
stimulated to ovulate with PMSG/human chorionic gonadotrophin (HCG) were
incubated for 30 min in similar concentrations of Propofol prior to IVF.
Maturation, fertilization and cleavage rates were compared. A significant
decrease in the in-vitro maturation rate was observed only when the
cumulus-free and cumulus-enclosed oocytes were exposed to 10,000 ng/ml
Propofol (P < 0.0074 and P < 0.0001 respectively). Fertilization and
embryo cleavage rates were not significantly different compared with the
controls. These findings give some reassurance with respect to human IVF.
However, further studies on the potential effects of Propofol on
implantation and pregnancy outcome following IVF are needed.
ARTICLES
The effect of the anaesthetic, Propofol, on in-vitro oocyte maturation, fertilization and cleavage in mice
Department of Obstetrics and Gynaecology, University Hospital, Nottingham, UK.
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