Human Reproduction, Vol 12, 1525-1530, Copyright © 1997 by Oxford University Press
GM Warnes, D Payne, R Jeffrey, L Hourigan, C Kirby, J Kerin and C Matthews
Over a 26 month period 17% of couples having treatment in our clinical
programmes selected a commercially available protein (normal serum albumin,
NSA) prepared from pooled human sera instead of using their own serum as a
supplement for their embryo culture media. In a retrospective analysis of
>2000 gonadotrophin-stimulated cycles and 1000 cycles where
frozen/thawed embryos were transferred, fertilization, embryo quality and
pregnancy rates following in-vitro fertilization (IVF), gamete
intra-Fallopian transfer (GIFT) or intracytoplasmic sperm injection (ICSI)
were unaffected by the type of protein used to supplement the culture
medium. When embryos were thawed in medium containing NSA, both pregnancy
(PR) and implantation rates (IR) were significantly lower (P <0.05) than
if the medium was supplemented with serum (PR 8.3% and 17.5%; IR 4.6% and
10.5%). Inclusion of NSA before freezing reduced the IR of thawed embryos.
To further test this observation all cycles where embryos were cultured and
frozen in medium containing NSA (173 cycles) were matched to cycles where
serum was used and the outcome was compared. At the end of 1995 just over
half of the embryos in both groups had been thawed. No statistical
difference was noted in the pregnancy rates (NSA, 5.6% versus serum, 11.3%)
but the IR per embryo was significantly lower when embryos were cultured
and frozen in medium supplemented with NSA (2.2%) than when serum was used
as the supplement (6.6%).
ARTICLES
Reduced pregnancy rates following the transfer of human embryos frozen or thawed in culture media supplemented with normal serum albumin
The Queen Elizabeth Hospital, Woodville, South Australia.
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