Human Reproduction, Vol 12, 1537-1544, Copyright © 1997 by Oxford University Press
HY Huang, JS Krussel, Y Wen and ML Polan
In this study, we report a total of 292 mouse embryos cultured on Vero cell
monolayers and 77 embryos cultured in medium alone at different
preimplantation stages examined individually for embryonic mRNA of beta-
actin, interleukin-1beta (IL-1beta), interleukin-1 receptor antagonist
(icIL-1ra) and interleukin-1 receptor type I (IL-1RtI) using reverse
transcription and two-step polymerase chain reaction (RT-PCR). The rates of
blastocyst formation and blastocyst hatching were both significantly higher
in embryos co-cultured with Vero cells in comparison with the embryos
cultured in control medium (81.2 +/- 2.6 versus 42.2 +/- 3.7%, P <
0.001; 75.6 +/- 2.7 versus 19.2 +/- 6.2%, P < 0.001 respectively). We
have identified a similar pattern of interleukin-1 family embryonic mRNA
transcripts expressed from the compact morula stage through to hatching
blastocyst in both control and Vero cell cultured embryos with
significantly increased icIL-1ra transcript at hatching blastocyst stage (P
< 0.05, P < 0.001 respectively). There was a significant increase in
IL-1beta mRNA transcripts of embryos at hatching blastocyst stage compared
to compact morula stage in Vero cell cultured embryos (P < 0.05). These
findings support the hypothesis that the IL-1 system is an important factor
in embryo-maternal molecular communication during the implantation process.
ARTICLES
Use of reverse transcription-polymerase chain reaction to detect embryonic interleukin-1 system messenger RNA in individual preimplantation mouse embryos co-cultured with Vero cells
Department of Gynecology and Obstetrics, Stanford University Medical Center, California, USA.
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