Human Reproduction, Vol 12, 1663-1666, Copyright © 1997 by Oxford University Press
A Mansat, C Mengelle, M Chalet, A Boumzebra, R Mieusset, J Puel, C Prouheze and M Segondy
The presence of cytomegalovirus (CMV) in semen was retrospectively
evaluated in 178 cryopreserved semen samples obtained from 97 donors
recruited by two sperm banks. Thirty-three (34.0%) donors were CMV-
seronegative, 51 (52.6%) were CMV-seropositive and 13 (13.4%) had an
unknown serological status. CMV was detected in sperm specimens by cell
culture and polymerase chain reaction (PCR). CMV was detected in five
(2.8%) samples from two (2.0%) donors by cell culture and in 10 samples
(5.6%) from five donors (5.1%) by PCR; all the samples which were positive
by cell culture were also positive by PCR. Our data indicate that
cryopreserved semen specimens obtained from healthy volunteer donors
represent a potential source of CMV infection through therapeutic donor
insemination. PCR, which is more sensitive than cell culture, should be
used for direct and rapid identification of CMV in semen samples collected
from volunteer donors. CMV detection in semen by PCR could be
advantageously compared to CMV-antibody tests in serum which are only
indicative of a former CMV infection.
ARTICLES
Cytomegalovirus detection in cryopreserved semen samples collected for therapeutic donor insemination
Department of Urology and Andrology, CECOS Midi-Pyrenees, Hopital La Grave, Toulouse, France.
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