Human Reproduction, Vol 13, 182-187, Copyright © 1998 by Oxford University Press
D Sakkas, Y Shoukir, D Chardonnens, PG Bianchi and A Campana
In-vitro fertilization (IVF) embryos are selected for transfer on the basis
of morphology and rate of development. However, when a number of embryos
have similar characteristics, the selection of the best embryos is left to
chance. Recently, we proposed a simple, novel method to overcome this
problem, based on pre-selection of embryos cleaving early to the two-cell
stage. In this study we have adopted the same method to choose embryos
fertilized after intracytoplasmic sperm injection (ICSI). Fertilized
embryos that had cleaved to the two-cell stage by 27 h post-injection were
designated as 'early cleavage' embryos, while those that had not yet
reached the two-cell stage were designated as 'no early cleavage'. In all
cases, the early cleavage embryos were transferred when available. Early
cleavage was observed in 54 (61.4%) of the 88 cycles assessed. There were
significantly (P = 0.04) more clinical pregnancies in the early cleavage
group, 14/54 (25.9%), compared with the no early cleavage group 2/34
(3.2%). No differences between the groups were found when comparing key
parameters (age, stimulation protocol and semen characteristics) of the
couples. Using the ICSI technique, we have shown that early cleavage to the
two-cell stage is not influenced by the timing of fertilization, and is
more likely due to intrinsic factors within the oocyte or embryo that
promote embryo cleavage after fertilization.
ARTICLES
Early cleavage of human embryos to the two-cell stage after intracytoplasmic sperm injection as an indicator of embryo viability
Clinic of Infertility and Gynaecological Endocrinology-WHO Collaborating Centre in Human Reproduction, Department of Obstetrics and Gynaecology, University Hospital of Geneva, Switzerland.
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