Human Reproduction, Vol 13, 3156-3159, Copyright © 1998 by Oxford University Press
MJ Tucker, PC Morton, G Wright, CL Sweitzer and JB Massey
Clinical egg cryopreservation has been applied during a 4-year period with
some limited success. Mostly mature and a few immature eggs were frozen
slowly and thawed rapidly in 1,2-propanediol and sucrose, and subsequently
inseminated by intracytoplasmic sperm injection (ICSI). Three studies were
performed in which: (i) it was established that 55% of aged unfertilized
mature eggs survive freezing; (ii) in 22 cycles of thawed donated eggs
cryosurvival was 24% with 15 cycles reaching transfer, and five pregnancies
were initiated, one of which went to term at 39 weeks with fraternal twin
boys, and one remains ongoing at 37 weeks; and (iii) in five cycles, where
in-vitro fertilization patients had some of their own eggs frozen/ thawed,
cryosurvival of mature eggs was poor at only 2.2%, although 44% sibling
germinal vesicle (GV) stage eggs survived. A normal female infant delivered
at 40 weeks arose from transfer of two embryos where GV eggs underwent in-
vitro maturation post-thaw and were fertilized by ICSI. Pregnancies
reported here and by others indicate a burgeoning awareness of the
potential benefits of egg cryopreservation, prompting cautious optimism for
the future of this technology.
ARTICLES
Clinical application of human egg cryopreservation
Reproductive Biology Associates, Atlanta, Georgia 30342, USA.
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