Human Reproduction, Vol 13, 3469-3477, Copyright © 1998 by Oxford University Press
PH Leung, LA Salamonsen and JK Findlay
Inhibin/activin alphaC/alphaN and betaA subunits were localized
immunohistochemically in the human endometrium throughout the menstrual
cycle using an affinity-purified sheep polyclonal antibody raised against
the alphaC/alphaN subunit and an affinity-purified rabbit polyclonal
antibody raised against the betaA subunit. The betaB subunit was below the
level of detection in all human endometrial samples tested. Immunoreactive
inhibin alphaC/alphaN subunit was localized in the luminal epithelium,
glandular epithelium, stromal tissues and vascular endothelium with no
significant variation across the normal menstrual cycle. Immunoreactive
betaA subunit, common to inhibin A and activins AA and AB was localized in
the luminal and glandular epithelium and in migratory cells while the
endometrial stromal cells, decidua, vascular smooth muscle and endothelium
were devoid of immunoreactivity. A significant variation of immunoreactive
betaA subunit was observed in glandular and luminal epithelium across the
normal menstrual cycle. In proliferative endometrium, only a very low level
of betaA immunostaining was seen in luminal and glandular epithelium, while
the luminal epithelial staining increased significantly in the early
secretory phase and remained relatively constant over the rest of the
menstrual cycle. A progressive increase in betaA immunoreactivity was
observed also in the glandular epithelium during the secretory phase
reaching a maximum in the late secretory phases, and decreasing at
menstruation. Co-localization studies on serial sections suggested that the
migratory cells expressing strong betaA immunoreactivity were macrophages
and neutrophils but not eosinophils or mast cells. Thus, cells within the
human endometrium are capable of expressing inhibin/activin molecules in
vivo. The variation in the pattern of secretion of the betaA subunit across
the menstrual cycle suggests that activin peptides may have a physiological
role in endometrial function.
ARTICLES
Immunolocalization of inhibin and activin subunits in human endometrium across the menstrual cycle
Prince Henry's Institute of Medical Research, Clayton, Victoria, Australia.
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