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Human Reproduction, Vol 13, 709-714, Copyright © 1998 by Oxford University Press

ARTICLES

Ooplasmic injection of elongating spermatids for the treatment of non- obstructive azoospermia

NV Sofikitis, Y Yamamoto, I Miyagawa, G Mekras, Y Mio, T Toda, S Antypas, H Kawamura, N Kanakas, N Antoniou, D Loutradis, T Mantzavinos, K Kalianidis and E Agapitos
Reproductive Physiology and IVF Center, Department of Urology, Tottori University School of Medicine, Yonago, Japan.

We applied the technique of ooplasmic elongating spermatid injection to the treatment of non-obstructive azoospermia. Mature oocytes were injected with elongating spermatids isolated from testicular biopsy material obtained from 13 non-obstructed azoospermic men. Seventy-three oocytes were successfully injected with elongating spermatids and were then cultured for 36 h. At 13 h post-injection 68 oocytes were found to be activated and 52 of them were fertilized. Forty-one 2- to 4-cell stage embryos developed from normally fertilized oocytes were transferred. At least two embryos were transferred to each female partner. Two pregnancies were achieved. Elongating spermatid injection may have a role in the treatment of non-obstructive azoospermia.
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