Human Reproduction, Vol 13, 724-729, Copyright © 1998 by Oxford University Press
SH McKiernan and BD Bavister
Hamster one-cell embryos were collected from two groups of donors: females
that were super-stimulated with pregnant mare's serum gonadotrophin (PMSG)
and females that were not. Embryos were cultured for 72 h and scored for
development. Morulae and blastocysts from the PMSG- and non-stimulated
females were transferred into contralateral uterine horns of non-stimulated
recipient females, so that experimental embryos (from PMSG-stimulated
females) and control embryos (from non- stimulated females) were paired
within a single recipient. Right and left uterine horns of recipient
females were examined 11 days later for the number of implantation sites
and fetuses. After 72 h of culture, development to morulae and blastocysts
was not significantly different for embryos from PMSG- and non-stimulated
females. However, embryos from PMSG-stimulated females compared to controls
had significantly reduced mean cell numbers (18 versus 21; P=0.003) and a
two-fold decrease in viability post-transfer (20 versus 45%; P=0.02). These
findings indicate that gonadotrophin stimulation compromises subsequent
developmental competence either during oocyte maturation or in the very
early embryo, but it is unclear whether reduced viability is a direct
effect or is an indirect consequence of PMSG stimulation.
ARTICLES
Gonadotrophin stimulation of donor females decreases post-implantation viability of cultured one-cell hamster embryos
Department of Animal Health and Biomedical Sciences, University of Wisconsin, Madison 53706, USA.
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