Human Reproduction, Vol 13, 991-997, Copyright © 1998 by Oxford University Press
M Lane and DK Gardner
Culture of in-vivo-developed mouse blastocysts in a simple culture medium
based on a balanced salt solution supplemented with carbohydrates for 3 h
significantly perturbed embryo metabolism. Maximal perturbation occurred
after just 6 h of culture. Similarly, culture of rat blastocysts in a
simple culture medium for 3 h also resulted in perturbed metabolism.
Cultured mouse and rat blastocysts both had an abnormally elevated rate of
glycolysis of approximately 100% after culture (P < 0.05). Rates of
pyruvate oxidation by mouse blastocysts were also significantly reduced
after culture in a simple medium for 6 h (P < 0.01). Furthermore, the
developmental competence of mouse blastocysts after transfer was
significantly reduced by just 6 h of culture in a simple medium (P <
0.05). Addition of Eagle's amino acids or vitamins to the culture medium
reduced the perturbation of both the glycolytic activity and oxidative
capacity of cultured mouse blastocysts and acted in synergy to further the
inhibition. Importantly, culture with amino acids and vitamins prevented
any loss of viability of mouse blastocysts after culture for 6 h. It can be
concluded that the mouse blastocyst is sensitive to its environment and
that culture-induced stress results in the loss of normal cellular
function, as manifested in this case by an abnormal pattern of glucose
utilization and loss of viability.
ARTICLES
Amino acids and vitamins prevent culture-induced metabolic perturbations and associated loss of viability of mouse blastocysts
Institute of Reproduction and Development, Monash University, Clayton, Victoria, Australia.
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