Human Reproduction, Vol 13, 1584-1589, Copyright © 1998 by Oxford University Press
A Castro, O Castro, JL Troncoso, P Kohen, C Simon, M Vega and L Devoto
Flow cytometry analysis of luteal cells revealed that an important
proportion of these cells are leukocytes. The percentage of leukocytes was
higher in the early (42 +/- 4) and late (35 +/- 3) luteal phases than in
the mid-luteal (24 +/- 2) phase. However, the proportion of macrophages did
not differ between the luteal stages. The flow cytometric properties
correlated with cellular size and granularity were not reliable as
discriminators of luteal cell subpopulations. Therefore, to assess the
contribution of luteal leukocytes, these cells were completely removed from
luteal cell suspensions (total cells), by a negative selection procedure
(immunomagnetic separation). The functional role of leukocytes in
mid-luteal steroidogenesis was assessed, in total as well as
leukocyte-depleted cells. Progesterone production was found to have
increased 2.2-fold in leukocyte-depleted cell cultures, in comparison with
total cells under basal conditions. However, the response to human
chorionic gonadotrophin (HCG) was 36% lower under the latter conditions.
Oestradiol production was not significantly modified under basal or
HCG-treated conditions. In leukocyte-depleted cells, the concentration of
interleukin (IL)-1beta decreased 5-fold in comparison with total cell
cultures, suggesting that leukocytes are the principal source of IL-1beta.
In summary, the results of the present investigation suggest functional
interactions between the immune system and steroidogenic cells of the human
corpus luteum.
ARTICLES
Luteal leukocytes are modulators of the steroidogenic process of human mid-luteal cells
Institute of Maternal and Child Research, School of Medicine and Department of Obstetrics and Gynaecology, University of Chile, San Borja-Arriaran Clinical Hospital, National Health Service, Santiago.
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