Human Reproduction, Vol 13, 2548-2554, Copyright © 1998 by Oxford University Press
C Staessen, C Janssenswillen, E De Clerck and A Van Steirteghem
For many years, we have been using Menezo B2 medium in our human in- vitro
fertilization (IVF) programme. Menezo B2 contains bovine serum albumin,
which has in the meantime become less popular. The search for a valid
alternative has therefore been initiated. In a first study from December
1993 to June 1994, we compared Menezo B2 medium to Medi-Cult (Medi-Cult
Universal IVF medium). In this auto-controlled study, sibling oocytes
cultured in Menezo B2 showed a normal fertilization rate similar to that
for those cultured in Medi-Cult: 62.9 +/- 33.3 and 61.0 +/- 33.0%
respectively (NS; paired t-test). In a second auto- controlled study
conducted from November 1995 to March 1996, we compared Menezo B2 to BM1
(Ellios Bio-Media) medium. The normal fertilization rate in the sibling
oocytes was similar for those cultured in Menezo B2 and in BM1: 66.9 +/-
27.5 versus 62.0 +/- 31.7% (NS; paired t-test). After further culture of
the fertilized oocytes, no difference was apparent in either study as
regards the morphological characteristics of the embryos cultured in the
different media. The speed of development, however, did differ. In both
studies, we observed a higher proportion of the embryos reaching at least
the three- to four- cell stage at approximately 41 h post-insemination in
Menezo B2 medium than in either Medi-Cult or BM1. As regards the
implantation potency of the embryos obtained in the different media, data
were collected from the non-randomized transfers. These data indicate no
difference between the media tested. This study indicates that the three
commercial media with a different composition are equally able to sustain
fertilization and embryo development until transfer.
ARTICLES
Controlled comparison of commercial media for human in-vitro fertilization: Menezo B2 medium versus Medi-Cult universal and BM1 medium
Centre for Reproductive Medicine, University Hospital, Dutch-speaking Brussels Free University, Belgium.
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