Human Reproduction, Vol 13, 2555-2560, Copyright © 1998 by Oxford University Press
N Ogonuki, T Sankai, F Cho, K Sato and Y Yoshikawa
Intracytoplasmic sperm injection (ICSI) and partial zona dissection
followed by insemination (PZD-I) were used to establish a microinjection
system in cynomolgus monkeys (Macaca fascicularis), which are potential
models for human reproduction. Two experimental systems were studied, in
which either hamster oocytes or cynomolgus monkey oocytes were used as the
vehicle. When hamster oocytes were used, 66 out of 81 ICSI-treated oocytes
(82%) showed sperm head swelling or pronucleus formation. Following PZD-I
of hamster oocytes the rates of spermatozoa penetration (85/114; 75%) and
fertilization (71/114; 62%) were relatively high. When cynomolgus monkey
oocytes were used, 19 out of 31 (61%) were fertilized by ICSI with
cynomolgus monkey spermatozoa and, subsequently, two embryos (7%) developed
to the morula stage. In total, 94% (15/16) of the PZD-I treated oocytes
were penetrated by spermatozoa and 63% (10/16) were fertilized. These
results demonstrate that both micromanipulation techniques can be used in
assisted fertilization with cynomolgus monkeys.
ARTICLES
Comparison of two methods of assisted fertilization in cynomolgus monkeys (Macaca fascicularis): intracytoplasmic sperm injection and partial zona dissection followed by insemination
Tsukuba Primate Center, The National Institute of Infectious Diseases, Ibaraki, Japan.
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