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Human Reproduction, Vol 13, 2568-2574, Copyright © 1998 by Oxford University Press

ARTICLES

Flow cytometric light scattering analysis, acrosome reaction, reactive oxygen species production and leukocyte contamination of semen preparation in prediction of fertilization rate in vitro

JM Moilanen, O Carpen and O Hovatta
Infertility Clinic, The Family Federation of Finland, Helsinki.

The effects on fertilization of the morphology of spermatozoa, acrosome reaction, reactive oxygen species (ROS) production, leukocyte contamination and the light scattering in flow cytometry of semen preparation were investigated in 73 couples undergoing their first in- vitro fertilization treatment. All men had normal concentrations of spermatozoa with sufficient motility (> or = 50%) and yield (> or = 6 x 10(6)) in the semen preparation on the day of oocyte retrieval. The light scattering properties of all cells present in the semen preparation, as assessed with flow cytometry, were correlated with fertilization. The proportion of metaphase I oocytes was also correlated with the fertilization rate of all collected oocytes and of metaphase II oocytes. ROS production, leukocyte contamination, spontaneous or calcium ionophore-stimulated acrosome reaction, percentage of normal morphology, and multiple anomalies index had no independent contribution.
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G. Ricci, G. Presani, S. Guaschino, R. Simeone, and S. Perticarari
Leukocyte detection in human semen using flow cytometry
Hum. Reprod., June 1, 2000; 15(6): 1329 - 1337.
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