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Human Reproduction, Vol. 14, No. 10, 2525-2530, October 1999
© 1999 European Society of Human Reproduction and Embryology

Dimeric inhibins and activin A in human follicular fluid and oocyte–cumulus culture medium

C.P. Lau1, W.L. Ledger1, N.P. Groome2, D.H. Barlow1 and S. Muttukrishna1,3

1 Nuffield Department of Obstetrics and Gynaecology, University of Oxford, John Radcliffe Hospital and 2 School of Biological and Molecular Sciences, Oxford Brookes University, Oxford, UK

The concentrations of inhibin A, inhibin B and activin A in follicular fluid and oocyte culture medium were analysed to investigate the production of these peptide hormones by ovarian granulosa cells and oocyte–cumulus complexes, as well as their potential as possible biochemical markers for oocyte quality and fertilizing capacity. Follicular fluids were collected from individual follicles during oocyte retrieval for in-vitro fertilization (IVF). Oocyte–cumulus culture media were collected after in-vitro insemination. The concentrations of dimeric inhibin A, inhibin B and activin A were measured using two-site enzyme-linked immunosorbent assays in the follicular fluid and matched oocyte culture medium. Hormone concentrations were compared with oocyte quality and fertilizing capacity. The concentration of inhibin A in follicular fluid increased while that of inhibin B decreased with increasing follicle size. Follicular fluid concentrations of inhibin A inhibin B and activin A were not significantly different in follicles with differing oocyte quality. Oocyte culture medium concentrations of activin A were significantly higher in morphologically good quality oocytes. There was no relationship between the concentrations of the three hormones and oocyte fertilizing capacity. This study confirms that follicular fluid concentrations of inhibin A may prove to be a marker of follicular growth and maturation. Higher concentrations of activin A produced by good quality oocyte–cumulus complexes suggest that activin A may play a role in oocyte maturation.

Key words: activin A/follicular fluids/inhibin A/inhibin B/oocytes

3 To whom correspondence should be addressed


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