Human Reproduction, Vol. 14, No. 11, 2908-2916,
November 1999
© 1999 European Society of Human Reproduction and Embryology
Quantitative near-infrared spectroscopy of cervical dysplasia in vivo
1 Laser Microbeam and Medical Program (LAMMP), Beckman Laser Institute and Medical Clinic, University of California (Irvine), 1002 Health Sciences Road East, Irvine, CA 92612, USA, 2 Departement Frauenheilkunde, Universitätsspital Zurich, Switzerland 3 Division of Gynecological Oncology, Medical Center, University of California, Irvine, CA, USA
The aims of this study were: (i) to quantify near-infrared optical properties of normal cervical tissues and high-grade squamous intra-epithelial lesions (H-SIL); (ii) to assess the feasibility of differentiating normal cervical tissues from H-SIL on the basis of these properties; and (iii) to determine how cervical tissue optical properties change following photodynamic therapy (PDT) of H-SIL in vivo. Using the frequency domain photon migration technique, non-invasive measurements of normal and dysplastic ecto-cervical tissue optical properties, i.e. absorption (µa) and effective scattering coefficients, and physiological parameters, i.e. tissue water and haemoglobin concentration, percentage oxygen saturation (%SO2), were performed on 10 patients scheduled for PDT of histologically-proven H-SIL. Cervix absorption and effective scattering parameters were up to 15% lower in H-SIL sites compared with normal cervical tissue for all wavelengths studied (674, 811, 849, 956 nm). Following PDT, all µa values increased significantly, due to elevated tissue blood and water content associated with PDT-induced hyperaemia and oedema. Tissue total haemoglobin concentration ([TotHb]) and arterio-venous oxygen saturation measured in H-SIL sites were lower than normal sites ([TotHb]: 88.6 ± 35.8 µmol/l versus 124.7 ± 22.6 µmol/l; %SO2: 76.5 ± 14.7% versus 84.9 ± 3.4%).
Key words: cervix/optical properties/frequency-domain photon migration/haemoglobin spectroscopy/photodynamic therapy
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