Human Reproduction, Vol. 14, No. 12, 2941-2943,
December 1999
© 1999 European Society of Human Reproduction and Embryology
Opinions |
Sperm cryopreservation: is there a significant risk of cross-contamination?
Andrology Department, The Royal Women's Hospital, Carlton, Victoria 3053, Australia
Interest in the subject of sperm cryopreservation has a long history, with thousands of published articles in the scientific/medical literature. Clinical pregnancy rates using donor insemination can now approach natural pregnancy rates (Clarke et al., 1997b ). However, an issue which has received very little attention in the literature is the subject of microbiological cross-contamination in sperm banks. This subject obviously concerns many sperm bank managers, who have instituted several approaches aimed at reducing the perceived risks. One approach is to screen all patients or donors for human immunodeficiency virus (HIV) status, hepatitis B and C and syphilis prior to cryopreservation of their semen. Another approach involves the use of quarantine tanks to hold samples until screening results are obtained, before placing the cleared samples into the main storage system. The samples of patients with positive screening results are then transferred to specially designated tanks. Both of these approaches
Sealing of straws or vials
Liquid nitrogen vapour storage
Other recommended precautions
Conclusions
Acknowledgments
Notes
References
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