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Human Reproduction, Vol. 14, No. 12, 2987-2990, December 1999
© 1999 European Society of Human Reproduction and Embryology

Preliminary study of the incidence of disomy in sperm fractions after MicroSort flow cytometry

F. Vidal1,4, J. Blanco1, E.F. Fugger2,3, K. Keyvanfar2, M. Norton2, J.D. Schulman2,3 and J. Egozcue1

1 Unitat de Biologia Cellular, Facultat de Ciències, Universitat Autònoma Barcelona, 08193-Barcelona, Spain, 2 Genetics and IVF Institute, Fairfax and 3 Medical College of Virginia, Richmond, VA, USA

Using triple colour fluorescent in-situ hybridization (FISH) we have evaluated, on a blind basis, the disomy level for chromosome 21 and the sex chromosomes in flow cytometric sorted sperm samples. There were no statistically significant differences in the disomy rates when comparing the sorted samples (either for X- or Y-bearing spermatozoa) with non-sorted samples. There were no diploid spermatozoa observed in any sample group after MicroSort® processing.

Key words: chromosome 21 disomies/flow cytometry/fluorescence in-situ hybridization/sperm sex selection

4 To whom correspondence should be addressed


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S. Egozcue, J. Blanco, F. Vidal, and J. Egozcue
Diploid sperm and the origin of triploidy
Hum. Reprod., January 1, 2002; 17(1): 5 - 7.
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