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Human Reproduction, Vol. 14, No. 2, 476-478, February 1999
© 1999 European Society of Human Reproduction and Embryology

Case Report: Intracytoplasmic sperm injection into zona-free human oocytes results in normal fertilization and blastocyst development

J. Ding1, N. Rana and W.P. Dmowski

Oak Brook Fertility Center, Oak Brook, Illinois 60523, USA

Zona-free human oocytes are frequently encountered in in-vitro fertilization (IVF) laboratories. The oocytes escape out of the zona pellucida, following zona fracture, which can occur during oocyte retrieval or manipulation, but occasionally may be the result of increased zona fragility. Some of the zona-free oocytes are mature and morphologically healthy; nevertheless, all are typically discarded. In this report, we demonstrate that zona-free oocytes can be fertilized normally using intracytoplasmic sperm injection (ICSI) and can subsequently develop without zona to the blastocyst stage in vitro. We therefore suggest that those mature and morphologically normal zona-free oocytes may be rescued, fertilized with ICSI and then cultured to the blastocyst stage for subsequent transfer or cryopreservation.

Key words: blastocyst formation/ICSI/zona-free oocytes/zona-free zygotes

1 To whom correspondence should be addressed at: 2425 W. 22nd Street, Suite 102, Oak Brook, IL 60523, USA


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