Human Reproduction, Vol. 14, No. 3, 726-730,
March 1999
© 1999 European Society of Human Reproduction and Embryology
Fertility of ejaculated and testicular megalohead spermatozoa with intracytoplasmic sperm injection
1 Sevgi Hospital, Assisted Reproductive Techniques and Reproductive Endocrinology Unit, Ankara, 2 Ankara University, Medical School, Department Of Histology and Embryology, Ankara, Turkey and 3 SIMAF, Van Helmont Hospital, Fertility Unit, Vilvoorde, Belgium
In this study the fertility and outcome of intracytoplasmic sperm injection (ICSI) using megalohead spermatozoa from the ejaculates and testicles was evaluated. Seventeen males with megalohead and pinhead sperm forms in their ejaculate were studied in 22 cycles. A high number of sperm heads without tails and abundant round spermatid forms were commonly observed. Round-headed spermatozoa were seldom accompanied by these severely abnormal spermatozoa. The majority of megalohead spermatozoa were observed to have multiple tails, were predominant in the sample, and were used for ICSI. Ejaculated megalohead spermatozoa were used for ICSI in 15 cycles, while testicular spermatozoa were used in seven cycles where there were no vital spermatozoa or spermatozoa of low vitality in the ejaculate. The same abnormal morphology was observed in the testicles as in the ejaculated spermatozoa in the same males. Mean (± SD) low motility 4.7 ± 5.6% and sperm count (3.8 ± 4.19x106) were common findings in these severely teratozoospermic patients. A low fertilization rate (43.2%) was achieved by using megalohead sperm forms (group I, n = 17) in comparison with the control group (60.2%) which had zero normal sperm morphology according to strict criteria (group II, n = 30) (P <0.01). Furthermore, a low pregnancy rate (9.1%) was obtained in the megalohead sperm group in comparison with the control group (40%) (P <0.05). Low fertilization and pregnancy rates may be due to a high incidence of chromosomal abnormalities from severely defective spermatozoa in the ejaculate. Couples should be counselled and warned about possible low fertilization and pregnancy rates with ICSI when only pinhead and megalohead forms with a high number of sperm heads without tails are present in the ejaculate.
Key words: intracytoplasmic sperm injection/megalohead/pinhead spermatozoa
4 To whom correspondence should be addressed at: Sevgi Hospital, Department of Reproductive Endocrinology and Infertility, Tunus Cad. No. 28, Kavaklidere, Ankara 06680, Turkey
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  K. Dieterich, R. Zouari, R. Harbuz, F. Vialard, D. Martinez, H. Bellayou, N. Prisant, A. Zoghmar, M. R. Guichaoua, I. Koscinski, et al. The Aurora Kinase C c.144delC mutation causes meiosis I arrest in men and is frequent in the North African population Hum. Mol. Genet., April 1, 2009; 18(7): 1301 - 1309. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. Achard, O. Paulmyer-Lacroix, G. Mercier, G. Porcu, J. Saias-Magnan, C. Metzler-Guillemain, and M. R. Guichaoua Reproductive Failure in Patients With Various Percentages of Macronuclear Spermatozoa: High Level of Aneuploid and Polyploid Spermatozoa J Androl, July 1, 2007; 28(4): 600 - 606. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Donoso, C. Staessen, B.C.J.M. Fauser, and P. Devroey Current value of preimplantation genetic aneuploidy screening in IVF Hum. Reprod. Update, January 1, 2007; 13(1): 15 - 25. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Berkovitz, F. Eltes, A. Ellenbogen, S. Peer, D. Feldberg, and B. Bartoov Does the presence of nuclear vacuoles in human sperm selected for ICSI affect pregnancy outcome? Hum. Reprod., July 1, 2006; 21(7): 1787 - 1790. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. C. Juneja and J. M. van Deursen A mouse model of familial oligoasthenoteratozoospermia Hum. Reprod., April 1, 2005; 20(4): 881 - 893. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Vicari, A. De Palma, N. Burrello, G. Longo, C. Grazioso, N. Barone, M. Zahi, R. D'agata, and A. E. Calogero Absolute Polymorphic Teratozoospermia in Patients With Oligo-Asthenozoospermia Is Associated With an Elevated Sperm Aneuploidy Rate J Androl, July 1, 2003; 24(4): 598 - 603. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Kahraman, Y. Kumtepe, S. Sertyel, E. Donmez, M. Benkhalifa, N. Findikli, and P. Vanderzwalmen Pronuclear morphology scoring and chromosomal status of embryos in severe male infertility Hum. Reprod., December 1, 2002; 17(12): 3193 - 3200. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Escalier Genetic approach to male meiotic division deficiency: the human macronuclear spermatozoa Mol. Hum. Reprod., January 1, 2002; 8(1): 1 - 7. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Yakin and S. Kahraman Certain forms of morphological anomalies of spermatozoa may reflect chromosomal aneuploidies Hum. Reprod., August 1, 2001; 16(8): 1779 - 1780. [Full Text] [PDF]
|
|
|
|
|
|
K. Steger, K. Failing, T. Klonisch, H. M. Behre, M. Manning, W. Weidner, L. Hertle, M. Bergmann, and S. Kliesch Round spermatids from infertile men exhibit decreased protamine-1 and -2 mRNA Hum. Reprod., April 1, 2001; 16(4): 709 - 716. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Viville, R. Mollard, M.-L. Bach, C. Falquet, P. Gerlinger, and S. Warter Do morphological anomalies reflect chromosomal aneuploidies?*: Case Report Hum. Reprod., December 1, 2000; 15(12): 2563 - 2566. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Kahraman, K. Yakin, E. Donmez, H. Samli, M. Bahce, G. Cengiz, S. Sertyel, M. Samli, and N. Imirzalioglu Relationship between granular cytoplasm of oocytes and pregnancy outcome following intracytoplasmic sperm injection Hum. Reprod., November 1, 2000; 15(11): 2390 - 2393. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Kahraman, M. Bahce, H. Samli, N. Imirzalioglu, K. Yakisn, G. Cengiz, and E. Donmez Healthy births and ongoing pregnancies obtained by preimplantation genetic diagnosis in patients with advanced maternal age and recurrent implantation failure Hum. Reprod., September 1, 2000; 15(9): 2003 - 2007. [Abstract] [Full Text] [PDF]
|
|