Human Reproduction, Vol. 14, No. 4, 1050-1056,
April 1999
© 1999 European Society of Human Reproduction and Embryology
Status of genomic imprinting in mouse spermatids
1 Reproductive Genetics Unit, Department of Obstetrics, Gynecology and Reproductive Sciences, Box 0720, University of California, San Francisco, CA 94143 and 2 Department of Anatomy and Reproductive Biology, University of Hawaii School of Medicine, Honolulu, Hawaii, 96822 USA
The advent of human round spermatid microinjection (ROSI) into oocytes as a treatment for severe male infertility raises the question of whether spermatids have undergone all of the maturation processes necessary for normal development. It is particularly important to know whether spermatids have undergone correct genomic imprinting, which results in the parent-of-origin-specific expression of only one allele of a gene. We assessed the imprinting status of three maternally and three paternally expressed genes in interspecific hybrid embryos generated by injecting Mus castaneus spermatids into Mus musculus oocytes. We used the single nucleotide primer extension (SNuPE) assay to measure the relative expression of maternal and paternal alleles on the basis of sequence polymorphisms in the transcripts. Expression of imprinted genes in mouse embryos derived by ROSI did not differ from controls, indicating that paternal genes have undergone proper imprinting by the round spermatid stage.
Key words: genomic imprinting/infertility/ROSI/spermatids
3 To whom correspondence should be addressed
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