Human Reproduction, Vol. 14, No. 4, 1096-1100,
April 1999
© 1999 European Society of Human Reproduction and Embryology
Parturition at term: parallel increases in interleukin-8 and proteinase concentrations and neutrophil count in the lower uterine segment
1 Department of Obstetrics and Gynaecology and 2 Institute of Clinical Chemistry and Pathobiochemistry, University Hospital, Technical University (RWTH), 52057 Aachen, 3 Institute of Pathology, University of Tübingen, 72076 Tübingen and 4 Institute of Biochemistry, University of Bielefeld, 33615 Bielefeld, Germany
A relationship was sought between the tissue concentrations of interleukin (IL)-8, matrix metalloproteinase (MMP)-8 and MMP-9, and the numbers of the various leukocytes infiltrating the lower uterine segment stroma during parturition. Biopsy specimens of the lower uterine segment were obtained from 63 women undergoing Caesarean section at various stages of cervical dilatation at term. The concentrations of IL-8, MMP-8 and MMP-9 were determined with enzyme-linked immunosorbent assays, and the leukocytes were quantified immunohistochemically. The median IL-8 concentration (pg/mg total protein) rose significantly from 17.2 at <2 cm dilatation, to 26.5 at 2 to <4 cm dilatation, and 1954.0 at 4–6 cm dilatation, and remained at approximately this concentration at >6 cm dilatation. The median MMP-8 concentration (ng/mg total protein) increased significantly from 32.2 at <2 cm dilatation to 114.2 at >6 cm dilatation. The median MMP-9 concentration (ng/mg total protein) rose significantly from 15.4 at <2 cm dilatation to 102.1 at >6 cm dilatation. The number of neutrophils was significantly higher at 4–6 cm and >6 cm dilatation than at >2 cm, reaching maximum values at >6 cm dilatation. The findings in this study support the hypothesis that IL-8-induced infiltration of the cervical stroma by neutrophils and subsequent release of proteinases may play a key role in parturition.
Key words: cervix uteri/interleukin-8/matrix metalloproteinases/neutrophils/parturition
5 To whom correspondence should be addressed at: Frauenklinik für Gynäkologie und Geburtshilfe, Universitätsklinikum der RWTH Aachen, Pauwelsstr. 30, D-52057 Aachen, Germany
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