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Human Reproduction, Vol. 14, No. 6, 1588-1592, June 1999
© 1999 European Society of Human Reproduction and Embryology

Evaluation of the effect of interleukin-6 and human extracellullar matrix on embryonic development

N. Desai1, M. Scarrow, J. Lawson, D. Kinzer and J. Goldfarb

Department of Reproductive Biology, Univ. MacDonald Women's Hospital, Case Western Reserve University, 11100 Euclid Av., Cleveland, OH 44106, USA

Extracellular matrices and their associated growth factors can modulate the in-vitro growth of cells. In this study, the effects of culture substrata and the cytokine interleukin-6 (IL-6) on embryonic development were investigated. In-vitro fertilized mouse oocytes were pooled and randomly distributed amongst treatment groups. The test treatments were: (i) IL-6, at either 500 or 1000 pg/ml; (ii) human extracellular matrix (HECM) applied to organ culture dishes at either 5.0 or 10.0 µg/ml; and (iii) HECM and IL-6 combined. A total of 1285 embryos was evaluated. The effect of IL-6 on embryos was dose dependent. Treated embryos exhibited higher blastulation and hatching rates than untreated control embryos. Culture of embryos on human matrix proteins versus standard culture surfaces significantly improved in-vitro hatching. The combination of both of these treatments was superior to the medium alone control, and the mean cell count per blastocyst was higher (131.7 ± 29.7 versus 82.5 ± 14.3 in control embryos; P < 0.0001). In a pilot study with human triploid embryos, the HECM/IL-6 culture system appeared to support embryonic compaction, blastulation and hatching. This work suggests that extracellular matrix components in combination with growth factors/cytokines may be another avenue for formulating more physiological culture systems.

Key words: blastocyst/co-culture/extracellular matrix/growth factor/Matrigel

1 To whom correspondence should be addressed


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