Human Reproduction, Vol. 14, No. 9, 2191-2199,
September 1999
© 1999 European Society of Human Reproduction and Embryology
Positive outcome after preimplantation diagnosis of aneuploidy in human embryos *
1 Institute for Reproductive Medicine and Science, Saint Barnabas, Livingston, NJ, 2 S.I.S.Me.R, Bologna, Italy, 3 Reproductive Biology Associates, Atlanta, GA, USA
Chromosomal abnormalities are responsible for a great deal of embryo wastage, which is reflected, at least partially, in decreased implantation and increased miscarriage in older women. To address this problem the transfer of only chromosomally normal embryos previously selected by preimplantation genetic diagnosis (PGD) has been proposed. We designed a multi-centre in-vitro fertilization (IVF) study to compare controls and a test group that underwent embryo biopsy and PGD for aneuploidy. Patients were matched retrospectively, but blindly, for average maternal age, number of previous IVF cycles, duration of stimulation, oestradiol concentrations on day +1, and average mature follicles. All these parameters were similar in test and control groups. Only embryos classified as normal for those chromosomes were transferred after PGD. The results showed that the rates of fetal heart beat (FHB)/embryo transferred between the control and test groups were similar. However, spontaneous abortions, measured as FHB aborted/FHB detected, decreased after PGD (P < 0.05), and ongoing pregnancies and delivered babies increased (P < 0.05) in the PGD group of patients. Two conclusions were obtained: (i) PGD of aneuploidy reduced embryo loss after implantation; (ii) implantation rates were not significantly improved, but the proportion of ongoing and delivered babies was increased.
Key words: in-vitro fertilization/preimplantation genetic diagnosis/trisomy 21, 18, 13, 16
4 To whom correspondence should be addressed at: The Institute for Reproductive Medicine and Science, Saint Barnabas Medical Center,101 Old Short Hills Rd, Suite 501, Livingston, NJ 07052, USA
* Some of the data in this paper were presented as an abstract in the 54th Annual Meeting of The American Society of Reproductive Medicine (San Francisco, CA, USA, 1998), where it was designated the Prize Paper of the Society for Assisted Reproductive Technology.
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