Human Reproduction, Vol. 15, No. 1, 157-164,
January 2000
© 2000 European Society of Human Reproduction and Embryology
Culture of one-cell hamster embryos with water soluble vitamins: pantothenate stimulates blastocyst production
Department of Animal Health and Biomedical Sciences, School of Veterinary Medicine, University of Wisconsin, Madison, WI 53706, USA
The effects of water-soluble vitamins, singly or in combinations, on development of hamster 1-cell embryos were examined in a protein-free, chemically defined culture medium, HECM-6. Pantothenate significantly stimulated blastocyst development compared to the vitamin-free control and to every other single vitamin, except thiamine. Ascorbic acid, biotin, choline, folic acid, inositol, niacinamide, pyridoxal, riboflavin and thiamine had no detectable stimulation or inhibition on cleavage stage development or morula/blastocyst formation. When combinations of vitamins were tested, embryo development was either unchanged or significantly greater than in the control, but never significantly greater than development with pantothenate alone. A dose response to pantothenate indicated that 3 µmol/l was the optimum concentration. After embryo transfer, the percentage of live fetuses recovered per 100 1-cell embryos cultured in HECM-6 plus pantothenate (now designated HECM-9) was 24%, significantly higher than the 11% recovered from 100 1-cell embryos cultured in HECM-6 alone. This is the first report to show a stimulatory effect of a single vitamin on in-vitro development of preimplantation embryos in any mammalian species.
Key words: development/embryo/pantothenate/vitamins
1 To whom correspondence should be addressed
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  J. K. Nyholt de Prada, Y. S. Lee, K. E. Latham, C. L. Chaffin, and C. A. VandeVoort Role for cumulus cell-produced EGF-like ligands during primate oocyte maturation in vitro Am J Physiol Endocrinol Metab, May 1, 2009; 296(5): E1049 - E1058. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Dupont, B. D. Bavister, D. R. Armant, and C. A. Brenner Rhesus macaque embryos derived from MI oocytes maturing after retrieval display high rates of chromosomal anomalies Hum. Reprod., April 1, 2009; 24(4): 929 - 935. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. S. Lee, K. E. Latham, and C. A. VandeVoort Effects of in vitro maturation on gene expression in rhesus monkey oocytes Physiol Genomics, October 8, 2008; 35(2): 145 - 158. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Takenaka, T. Horiuchi, and R. Yanagimachi Effects of light on development of mammalian zygotes PNAS, September 4, 2007; 104(36): 14289 - 14293. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S.M. Mitalipov, Q. Zhou, J.A. Byrne, W.Z. Ji, R.B. Norgren, and D.P. Wolf Reprogramming following somatic cell nuclear transfer in primates is dependent upon nuclear remodeling Hum. Reprod., August 1, 2007; 22(8): 2232 - 2242. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Sugihara, D. Sugiyama, J. Byrne, D. P. Wolf, K. P. Lowitz, Y. Kobayashi, M. Kabir-Salmani, D. Nadano, D. Aoki, S. Nozawa, et al. Trophoblast cell activation by trophinin ligation is implicated in human embryo implantation PNAS, March 6, 2007; 104(10): 3799 - 3804. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Q. Zhou, S.H. Yang, C.H. Ding, X.C. He, Y.H. Xie, T.B. Hildebrandt, S.M. Mitalipov, X.H. Tang, D.P. Wolf, and W.Z. Ji A comparative approach to somatic cell nuclear transfer in the rhesus monkey Hum. Reprod., October 1, 2006; 21(10): 2564 - 2571. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. C. Enders, S. Meyers, C. A. VandeVoort, and G. C. Douglas Interactions of macaque blastocysts with epithelial cells in vitro Hum. Reprod., November 1, 2005; 20(11): 3026 - 3032. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Final Report of the Safety Assessment of Niacinamide and Niacin International Journal of Toxicology, September 1, 2005; 24(5_suppl): 1 - 31. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Zheng, B. Patel, M. McMenamin, E. Moran, A. M. Paprocki, M. Kihara, R. D. Schramm, and K. E. Latham Effects of Follicle Size and Oocyte Maturation Conditions on Maternal Messenger RNA Regulation and Gene Expression in Rhesus Monkey Oocytes and Embryos Biol Reprod, April 1, 2005; 72(4): 890 - 897. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D.P. Wolf, S. Thormahlen, C. Ramsey, R.R. Yeoman, J. Fanton, and S. Mitalipov Use of Assisted Reproductive Technologies in the Propagation of Rhesus Macaque Offspring Biol Reprod, August 1, 2004; 71(2): 486 - 493. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Haigo, Y. Yamauchi, F. Yazama, R. Yanagimachi, and T. Horiuchi Full-Term Development of Hamster Embryos Produced by Injection of Round Spermatids into Oocytes Biol Reprod, July 1, 2004; 71(1): 194 - 198. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Zheng, B. Patel, M. McMenamin, S. E. Reddy, A. M. Paprocki, R. D. Schramm, and K. E. Latham The Primate Embryo Gene Expression Resource: A Novel Resource to Facilitate Rapid Analysis of Gene Expression Patterns in Non-Human Primate Oocytes and Preimplantation Stage Embryos Biol Reprod, May 1, 2004; 70(5): 1411 - 1418. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Zheng, B. Patel, M. McMenamin, A. M. Paprocki, R. D. Schramm, N. G. Nagl Jr, D. Wilsker, X. Wang, E. Moran, and K. E. Latham Expression of Genes Encoding Chromatin Regulatory Factors in Developing Rhesus Monkey Oocytes and Preimplantation Stage Embryos: Possible Roles in Genome Activation Biol Reprod, May 1, 2004; 70(5): 1419 - 1427. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. M. Mitalipov, H.-C. Kuo, J. D. Hennebold, and D. P. Wolf Oct-4 Expression in Pluripotent Cells of the Rhesus Monkey Biol Reprod, December 1, 2003; 69(6): 1785 - 1792. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R.D. Schramm, A. M. Paprocki, and C. A. VandeVoort Causes of developmental failure of in-vitro matured rhesus monkey oocytes: impairments in embryonic genome activation Hum. Reprod., April 1, 2003; 18(4): 826 - 833. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. J. Quintans, M. J. Donaldson, M. V. Bertolino, and R. S. Pasqualini Birth of two babies using oocytes that were cryopreserved in a choline-based freezing medium Hum. Reprod., December 1, 2002; 17(12): 3149 - 3152. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Yamauchi, R. Yanagimachi, and T. Horiuchi Full-Term Development of Golden Hamster Oocytes Following Intracytoplasmic Sperm Head Injection Biol Reprod, August 1, 2002; 67(2): 534 - 539. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Tatemoto, K. Ootaki, K. Shigeta, and N. Muto Enhancement of Developmental Competence after In Vitro Fertilization of Porcine Oocytes by Treatment with Ascorbic Acid 2-O-{alpha}-Glucoside During In Vitro Maturation Biol Reprod, December 1, 2001; 65(6): 1800 - 1806. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. E. Ludwig, M. Lane, and B. D. Bavister Differential Effect of Hexoses on Hamster Embryo Development in Culture Biol Reprod, May 1, 2001; 64(5): 1366 - 1374. [Abstract] [Full Text]
|
|
|
|
 |
|
 J. Zempleni, R. M. Helm, and D. M. Mock In Vivo Biotin Supplementation at a Pharmacologic Dose Decreases Proliferation Rates of Human Peripheral Blood Mononuclear Cells and Cytokine Release J. Nutr., May 1, 2001; 131(5): 1479 - 1484. [Abstract] [Full Text]
|
|
|
|
|
|
P. Zheng, H. Wang, B.D. Bavister, and W. Ji Maturation of rhesus monkey oocytes in chemically defined culture media and their functional assessment by IVF and embryo development Hum. Reprod., February 1, 2001; 16(2): 300 - 305. [Abstract] [Full Text] [PDF]
|
|