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Human Reproduction, Vol. 15, No. 11, 2285-2291, November 2000
© 2000 European Society of Human Reproduction and Embryology

Reactivity of different LH and FSH standards and preparations in the World Health Organization matched reagents for enzyme-linked immunoassays of gonadotrophins

A. Olivares1, M. Cárdenas2, C. Timossi3, T. Zariñán2,3, V. Díaz-Sánchez2 and A. Ulloa-Aguirre2,3,4

1 Research Unit in Developmental Biology, Hospital de Pediatría, Centro Médico Nacional S. XXI, IMSS, 2 Department of Reproductive Biology, Instituto Nacional de la Nutrición SZ, 3 Research Unit in Reproductive Medicine, Hospital de Ginecobstetricia No. 4, IMSS, Mexico DF, Mexico

The immunoreactivity of various LH and FSH calibration standards and recombinant preparations in the enzyme-linked immunoassay (EIA) systems for gonadotrophins developed for the Special Programme of Research in Human Reproduction of the World Health Organization (WHO) were compared. The preparations tested included three LH and two FSH pituitary standards (calibrated against LH 80/552 and 68/40 and FSH 78/549 respectively) provided with the EIA or radioimmunoassay WHO matched reagent kits, the pituitary preparation LER-907, and recombinant human LH (rhLH) and FSH (rhFSH). Simultaneous curve fitting of the EIA dose-response curves revealed no significant differences among the slopes generated by the WHO LH standards and LER-907; in contrast, no parallelism was found between the curves of rhLH and the pituitary-derived LH standards. No significant differences were found among the slopes of the curves elicited by the pituitary and recombinant FSH preparations. Each LH preparation exhibited a high degree of charge heterogeneity. Considerable variations in charge isoform distribution among the WHO LH standards, rhLH and LER-907 were also evident. In contrast, the FSH preparations were less heterogeneous and exhibited minor differences in charge distribution. Despite the existing differences in charge isoform distribution, all the pituitary-derived preparations as well as rhFSH seem appropriate for using as calibration standards in this particular EIA system.

Key words: enzyme-linked immunoassay/FSH/LH

4 To whom correspondence should be addressed at: Research Unit in Reproductive Medicine, Instituto Mexicano del Seguro Social, Apdo. Postal 99–065, Unidad Independencia, Mexico 10101, DF, Mexico. E-mail: aulloaa{at}buzon.main.conacyt.mx


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