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Human Reproduction, Vol. 15, No. 2, 344-350, February 2000
© 2000 European Society of Human Reproduction and Embryology

The relationship between fertility potential measurements on cryobanked semen and fecundity of sperm donors

T. Navarrete1,3,4, A. Johnson1, B. Mixon1 and D. Wolf1,2,3,5

1 1Department of Obstetrics/Gynecology, Andrology/Embryology Laboratory, Oregon Health Sciences University, 2 Department of Physiology/Pharmacology, Oregon Health Sciences University, Portland, OR 97201 and 3 Division of Reproductive Sciences, Oregon Regional Primate Research Center, Beaverton, OR 97006, USA

Sperm penetration assay (SPA) scores obtained from cryobanked semen were correlated with therapeutic insemination (TI) fecundity in a group of established sperm donors, thereby evaluating the efficacy of the SPA in screening donors for sperm banking. While the SPA has been used to separate fertile from infertile males, we altered assay conditions to use frozen semen and to distinguish performance among fertile donors. Three frozen ejaculates from 11 pregnancy-proven donors were analysed. Of 905 TI cycles, 275 recipients achieved 95 pregnancies. There were no significant relationships between fecundity and donor semen, washed sperm parameters, sperm recoveries or recipient age. A significant relationship was revealed between mean SPA scores (range 8.7–66.6 penetrations/ovum) and donor fecundity (range 0.04–0.16, P < 0.03). Sperm concentration was varied in an effort to establish the most sensitive test condition. Using 0.25x106 motile spermatozoa/ml, a highly significant relationship was observed (P < 0.002). The four donors with the lowest SPA scores achieved the four lowest fecundities. It is concluded that a modified SPA can be used on frozen donor semen to estimate donor fertility potential. If applied routinely in donor semen banking, poor quality applicants could be excluded, thereby increasing pregnancy rates while decreasing donor screening costs.

Key words: fecundity/frozen donor semen/sperm banking/sperm penetration assay/therapeutic insemination

4 Present address: Manizales 937, Lindavista, Mexico City, Mexico 07300 DF

5 To whom correspondence should be addressed at: Andrology/Embryology Laboratory, Oregon Health Sciences University,1750 S.W. Harbor Way, Suite 100, Portland, OR 97201-5164, USA


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