Human Reproduction, Vol. 15, No. 3, 557-566,
March 2000
© 2000 European Society of Human Reproduction and Embryology
Cell proliferation and vascular morphology in the marmoset corpus luteum
1 MRC Reproductive Biology Unit, Centre for Reproductive Biology, 37 Chalmers Street, Edinburgh EH3 9EW, UK, and 2 Department of Obstetrics and Gynaecology, Westmead Hospital, University of Sydney, NSW, Australia
Luteal formation is associated with angiogenesis and low progesterone production. Maximal mid-luteal phase progesterone production is concurrent with extensive vascularization, and luteolysis occurs when steroidogenesis decreases. Angiogenic cell proliferation and vascular changes have not been examined in the marmoset. The aim of this study was to examine vascular morphology throughout the luteal phase by identifying: (i) von Willebrand factor VIII antigen (vW)-immunopositive endothelial cells; (ii) Ki67-positive proliferating cells; and (iii) bromodeoxyuridine-positive proliferating cells. Marmoset corpora lutea were examined thoughout the cycle, and natural regression was compared with induced luteolysis after administration of a prostaglandin F2
analogue or gonadotrophin-releasing hormone (GnRH) antagonist. Steroidogenic and endothelial cells were positive for proliferation markers. Endothelial cell proliferation was highest during luteal formation, then decreased and remained low during the luteal phase and functional regression, however endothelial cell proliferation increased during structural regression. Endothelial cell proliferation was unchanged by induced regression. The area of vW immunostaining was highest during luteal formation, decreased thereafter and remained constant during the luteal phase and regression. Distribution of immunostaining indicated the presence of an extensive capillary network, but during structural regression the numbers of capillaries decreased and numbers of microvessels increased. These results suggest that vascular changes are concurrent with changes in the functional status of the marmoset corpus luteum.
Key words: angiogenesis/BrdU/corpus luteum/endothelial cells/Ki67
3 To whom correspondence should be addressed at: Department of Biotechnology, Flinders University of South Australia, Flinders Medical Centre, Bedford Park, Adelaide, South Australia
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  K.N. Fru, M. Cherian-Shaw, M. Puttabyatappa, C.A. VandeVoort, and C.L. Chaffin Regulation of granulosa cell proliferation and EGF-like ligands during the periovulatory interval in monkeys Hum. Reprod., May 1, 2007; 22(5): 1247 - 1252. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Sugino, T. Suzuki, A. Sakata, I. Miwa, H. Asada, T. Taketani, Y. Yamagata, and H. Tamura Angiogenesis in the Human Corpus Luteum: Changes in Expression of Angiopoietins in the Corpus Luteum throughout the Menstrual Cycle and in Early Pregnancy J. Clin. Endocrinol. Metab., November 1, 2005; 90(11): 6141 - 6148. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Kisliouk, H. Podlovni, K. Spanel-Borowski, O. Ovadia, Q.-Y. Zhou, and R. Meidan Prokineticins (Endocrine Gland-Derived Vascular Endothelial Growth Factor and BV8) in the Bovine Ovary: Expression and Role as Mitogens and Survival Factors for Corpus Luteum-Derived Endothelial Cells Endocrinology, September 1, 2005; 146(9): 3950 - 3958. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Kisliouk, N. Levy, A. Hurwitz, and R. Meidan Presence and Regulation of Endocrine Gland Vascular Endothelial Growth Factor/Prokineticin-1 and Its Receptors in Ovarian Cells J. Clin. Endocrinol. Metab., August 1, 2003; 88(8): 3700 - 3707. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. L. Chaffin, R. S. Brogan, R. L. Stouffer, and C. A. VandeVoort Dynamics of Myc/Max/Mad Expression during Luteinization of Primate Granulosa Cells in Vitro: Association with Periovulatory Proliferation Endocrinology, April 1, 2003; 144(4): 1249 - 1256. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. J. Rowe, K. D. Morris, R. Bicknell, and H. M. Fraser Angiogenesis in the Corpus Luteum of Early Pregnancy in the Marmoset and the Effects of Vascular Endothelial Growth Factor Immunoneutralization on Establishment of Pregnancy Biol Reprod, October 1, 2002; 67(4): 1180 - 1188. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Wulff, S. E. Dickson, W. C. Duncan, and H. M. Fraser Angiogenesis in the human corpus luteum: simulated early pregnancy by HCG treatment is associated with both angiogenesis and vessel stabilization Hum. Reprod., December 1, 2001; 16(12): 2515 - 2524. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. L. Chaffin, K. M. Schwinof, and R. L. Stouffer Gonadotropin and Steroid Control of Granulosa Cell Proliferation During the Periovulatory Interval in Rhesus Monkeys Biol Reprod, September 1, 2001; 65(3): 755 - 762. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Prange-Kiel, C. Kreutzkamm, U. Wehrenberg, and G. M. Rune Role of Tumor Necrosis Factor in Preovulatory Follicles of Swine Biol Reprod, September 1, 2001; 65(3): 928 - 935. [Abstract] [Full Text] [PDF]
|
|