Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF ) Freely available
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (11)
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Malik, E.
Right arrow Articles by Rück, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Malik, E.
Right arrow Articles by Rück, A.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Human Reproduction, Vol. 15, No. 3, 584-588, March 2000
© 2000 European Society of Human Reproduction and Embryology

Fluorescence diagnosis of endometriosis on the chorioallantoic membrane using 5-aminolaevulinic acid

E. Malik1,4, A. Meyhöfer-Malik1, Ch. Berg1, W. Böhm2, K. Kunzi-Rapp3, K. Diedrich1 and A. Rück3

1 Department of Obstetrics and Gynaecology, Medical University Lübeck, Ratzeburger Allee 160, D-23538 Lübeck, 2 Department of Obstetrics and Gynaecology, Medical University Ulm and 3 Institut für Lasertechnologien in der Medizin und Messtechnik, Ulm, Germany

The chorioallantoic membrane (CAM) is a useful model for the fluorescence diagnosis of experimentally induced endometriosis. In our experimental setup 75.7% of the histologically examined tissue preparations were viable and only 24.3% showed signs of necrosis on the CAM after various periods of incubation. Best results were obtained when grafting to the CAM was performed between days 7 and 9 and when implants were left on the CAM for 3–5 days (P < 0.05). We were able to demonstrate that 5-aminolaevulinic acid (ALA) is stored selectively in ectopic endometrium. The subsequent fluorescence of the endometrium shows a rapid increase that reaches a peak after 10–14 h which can be clearly differentiated from the weaker fluorescence of grafted normal peritoneum and fimbriae (P < 0.01).

Key words: 5-aminolaevulinic acid/chorioallantoic membrane/endometriosis/fluorescence diagnosis/photodynamic diagnosis

4 To whom correspondence should be addressed


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Hum Reprod UpdateHome page
R. Grummer
Animal models in endometriosis research
Hum. Reprod. Update, September 1, 2006; 12(5): 641 - 649.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.