Human Reproduction, Vol. 15, No. 4, 756-761,
April 2000
© 2000 European Society of Human Reproduction and Embryology
Secretory characteristics and viability of human term placental tissue after overnight cold preservation
1 Research Laboratory on Reproduction and 2 Laboratory of Pharmacology, Université Libre de Bruxelles (ULB), Brussels, Belgium and 3 Unité Vétérinaire, Université Catholique de Louvain (UCL), Louvain-La-Neuve, Belgium
Collection of human term placentae for research purposes is generally limited during working hours. Preserving placental tissue overnight might help to postpone experiments and, by extent, to increase material availability. In this study, fragments from normal placentae were incubated at 37°C either immediately after delivery or after preservation at 4°C in a HEPES-buffered solution or in a Roswell Park Memorial Institute (RPMI) 1640 culture medium. Protein, human chorionic gonadotrophin (HCG), human placental lactogen (HPL) and lactate dehydrogenase (LDH) contents within preserved explants were similar to those within freshly delivered ones. In contrast, HCG and HPL amounts released during incubation of preserved tissue were lower than with freshly delivered tissue. Differences were significant only during the first 3 h of incubation. Hormone releases were similarly Ca2+-stimulated, and Co2+- and low temperature-inhibited in preserved and freshly delivered tissues. After preservation, LDH leakage was also reduced. Furthermore, before and after 37°C incubation during 6 h, preserved tissue was morphologically indistinguishable from freshly delivered tissue and showed neither higher incidence of DNA fragmentation, nor elevated caspase-3 activity, both of which are markers of apoptosis. This study validates an original, useful and rapid method to preserve placental tissue. Consequently, this preservation model may facilitate the study of physiological processes regulating placental hormone secretion in normal and pathological conditions.
Key words: apoptosis/explant/placenta/release/viability
4 To whom correspondence should be addressed at: Research Laboratory on Reproduction, CPi 626, Faculty of Medicine, Université Libre de Bruxelles, 808, Route de Lennik, B-1070 Brussels, Belgium
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