Human Reproduction, Vol. 15, No. 4, 874-880,
April 2000
© 2000 European Society of Human Reproduction and Embryology
Post-implantation development of mouse androgenetic embryos produced by in-vitro fertilization of enucleated oocytes
1 Department of Animal Science, Tokyo University of Agriculture, Tokyo, Japan and 2 Department of Animal Breeding and Reproduction, Utsunomiya University, Tochigi, Japan
We report here on the precise ability of mouse androgenetic embryos produced by in-vitro fertilization of enucleated oocytes to develop to day 9.5 of gestation when cultured with M16 and CZB media. Androgenetic embryos cultured with CZB rather than M16 medium developed to the blastocyst stage in a more significant proportion (56.6% versus 45.0%, P < 0.001). However, after cavitation, the rate of cell proliferation of androgenetic embryos cultured with CZB medium was significantly decreased (P < 0.05). Embryo transfer experiments showed that blastocysts cultured with M16 medium were superior to those cultured with CZB medium in their ability to develop to 9.5-day-old fetuses (28.1% versus 11.1%, P < 0.001). These results showed that the present procedure for producing androgenetic mouse embryos is reliable and that M16 medium is superior for culturing the embryos. Fetal sexing by polymerase chain reaction (PCR) also demonstrated that both XX and XY embryos develop to 9.5-day fetuses at theoretical rates (1:2). This is the first finding that mouse XX androgenones survive after implantation.
Key words: androgenetic embryos/development/imprinting/mouse
3 Present address: Gene Research Center, Gunma University,3-39-22 Showa-machi, Maebashi, Gunma 371-8511, Japan
4 To whom correspondence should be addressed at: Department of Animal Science, Tokyo University of Agriculture, 1-1-1, Sakuragaoka, Setagaya-ku, Tokyo 156-8502, Japan
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  I. Lagutina, G. Lazzari, R. Duchi, and C. Galli Developmental Potential of Bovine Androgenetic and Parthenogenetic Embryos: A Comparative Study Biol Reprod, February 1, 2004; 70(2): 400 - 405. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Hernandez, S. Kozlov, G. Piras, and C. L. Stewart From The Cover: Paternal and maternal genomes confer opposite effects on proliferation, cell-cycle length, senescence, and tumor formation PNAS, November 11, 2003; 100(23): 13344 - 13349. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Obata and T. Kono Maternal Primary Imprinting Is Established at a Specific Time for Each Gene throughout Oocyte Growth J. Biol. Chem., February 8, 2002; 277(7): 5285 - 5289. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I Okamoto, S Tan, and N Takagi X-chromosome inactivation in XX androgenetic mouse embryos surviving implantation Development, January 10, 2000; 127(19): 4137 - 4145. [Abstract] [PDF]
|
|