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Human Reproduction, Vol. 15, No. 5, 1117-1120, May 2000
© 2000 European Society of Human Reproduction and Embryology

Enhancement of zona binding using 2-hydroxypropyl- ß-cyclodextrin

Jean Parinaud1,3, Gérard Vieitez1, Claude Vieu2, Xavier Collet2 and Bertrand Perret2

1 Laboratoire de Biologie de la Reproduction, CHU La Grave, 31052 Toulouse Cedex and 2 INSERM U326, CHU Purpan, Toulouse Cedex, France

Since membrane cholesterol depletion is known to play an important role in sperm capacitation, we have investigated the effect of 2-hydroxypropyl-ß-cyclodextrin, a cyclic oligosaccharide that mediates cholesterol efflux, on sperm functions. Sperm treatment with cyclodextrin did not affect the motility patterns but induced an increase in sperm binding to zona pellucida (24 ± 5 versus 13 ± 4 in control, P < 0.01). Cyclodextrin treatment was associated with an increase in spontaneous acrosome reaction (32 ± 8% versus 22 ± 4% in controls after a 4 h incubation, P < 0.05; 61 ± 10% versus 50 ± 11% in controls after a 24 h incubation, NS) but with a decrease in acrosome response to ionophore challenge (44 ± 5% versus 51 ± 3% in controls, P < 0.05). Concerning cell sterols, cyclodextrin induced a rapid and dramatic fall in the cholesterol and desmosterol content of spermatozoa. We conclude that cyclodextrin is a powerful capacitating agent, but since it induces an increase in spontaneous acrosome loss, it needs to be further evaluated before routine use in assisted reproductive technology media.

Key words: acrosome/cholesterol/cyclodextrin/spermatozoa/zona pellucida

3 To whom correspondence should be addressed


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