Human Reproduction, Vol. 15, No. 5, 1140-1148,
May 2000
© 2000 European Society of Human Reproduction and Embryology
Effect of recombinant human gonadotrophins on human, bovine and murine oocyte meiosis, fertilization and embryonic development in vitro
1 Monash Institute of Reproduction and Development, Monash Medical Center, 246 Clayton Road, Clayton, Victoria 3168, Australia and 2 Reproductive Medicine Unit, Societá Italinana per gli Studi sulla Medicina della Reproduzione srl, Via Mazzini 12, Bologna, Italy
The response of murine, bovine and human oocytes to pure recombinant preparations of human follicle stimulating hormone (rFSH) and luteinizing hormone (rLH) for meiotic maturation and subsequent developmental competence in vitro were examined in the present experiments. Maturation of immature bovine oocytes to the metaphase II stage was significantly increased by the addition of 1 IU/ml of rFSH in combination with either 1 IU/ml rLH or 10 IU/ml rLH. Similarly, embryonic development to the blastocyst stage was improved in bovine oocytes treated with a 1:10 combination of rFSH:rLH. However, no significant difference was observed in the number of inner cell mass or trophectoderm cells of the resulting blastocysts. Although the increased maturation to metaphase II was not significant, human embryonic developmental competence was improved by maturing oocytes in the presence of a 1:10 ratio of rFSH:rLH as only those oocytes exposed to a 1:10 ratio of rFSH: rLH during maturation showed normal cleavage patterns beyond day 2. In addition, 1 IU/ml rFSH and 1 IU/ml rLH increased the expression of oocyte proteins in human oocytes. The inclusion of recombinant gonadotrophins, either singly or in combination, had no significant effect on the maturation, fertilization or embryonic development of in-vitro matured mouse oocytes. These data provide support for the responsiveness of human and bovine oocytes to gonadotrophins in vitro and the need to consider variations in the relative concentrations for optimization of oocyte developmental competence.
Key words: embryonic development/immature oocytes/in-vitro maturation/oocyte proteins/recombinant gonadotrophins
3 To whom correspondence should be addressed
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  B. Heindryckx, P. De Sutter, J. Gerris, M. Dhont, and J. Van der Elst Embryo development after successful somatic cell nuclear transfer to in vitro matured human germinal vesicle oocytes Hum. Reprod., July 1, 2007; 22(7): 1982 - 1990. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. A. Silvestre, J. Alfonso, E. Garcia-Mengual, I. Salvador, C. C. Duque, and I. Molina Effect of recombinant human follicle-stimulating hormone and luteinizing hormone on in vitro maturation of porcine oocytes evaluated by the subsequent in vitro development of embryos obtained by in vitro fertilization, intracytoplasmic sperm injection, or parthenogenetic activation J Anim Sci, May 1, 2007; 85(5): 1156 - 1160. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. C. Chang, H. Liu, J. Zhang, J. Grifo, and L. C. Krey Developmental incompetency of denuded mouse oocytes undergoing maturation in vitro is ooplasmic in nature and is associated with aberrant Oct-4 expression Hum. Reprod., July 1, 2005; 20(7): 1958 - 1968. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 ESHRE Capri Workshop Group Fertility and ageing Hum. Reprod. Update, May 1, 2005; 11(3): 261 - 276. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Roberts, J. Stark, A. Iatropoulou, D. L. Becker, S. Franks, and K. Hardy Energy Substrate Metabolism of Mouse Cumulus-Oocyte Complexes: Response to Follicle-Stimulating Hormone Is Mediated by the Phosphatidylinositol 3-Kinase Pathway and Is Associated with Oocyte Maturation Biol Reprod, July 1, 2004; 71(1): 199 - 209. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Takeuchi, Z. Rosenwaks, and G. D. Palermo A successful model to assess embryo development after transplantation of prophase nuclei Hum. Reprod., April 1, 2004; 19(4): 975 - 981. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Hreinsson, B. Rosenlund, B. Friden, L. Levkov, I. Ek, A.-M. Suikkari, O. Hovatta, and M. Fridstrom Recombinant LH is equally effective as recombinant hCG in promoting oocyte maturation in a clinical in-vitro maturation programme: a randomized study Hum. Reprod., October 1, 2003; 18(10): 2131 - 2136. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Cukurcam, C. Hegele-Hartung, and U. Eichenlaub-Ritter Meiosis-activating sterol protects oocytes from precocious chromosome segregation Hum. Reprod., September 1, 2003; 18(9): 1908 - 1917. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. P. Cheung, S. M. Pride, B. H. Yuen, and L. Sy In-vivo ovarian androgen responses to recombinant FSH with and without recombinant LH in polycystic ovarian syndrome Hum. Reprod., October 1, 2002; 17(10): 2540 - 2547. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C.M.H. Combelles, N.A. Cekleniak, C. Racowsky, and D.F. Albertini Assessment of nuclear and cytoplasmic maturation in in-vitro matured human oocytes Hum. Reprod., April 1, 2002; 17(4): 1006 - 1016. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W.-Y. Son, S.-H. Yoon, S.-W. Lee, Y. Ko, H.-G. Yoon, and J.-H. Lim Blastocyst development and pregnancies after IVF of mature oocytes retrieved from unstimulated patients with PCOS after in-vivo HCG priming: Case report Hum. Reprod., January 1, 2002; 17(1): 134 - 136. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Wrenzycki, D. Herrmann, L. Keskintepe, A. Martins Jr, S. Sirisathien, B. Brackett, and H. Niemann Effects of culture system and protein supplementation on mRNA expression in pre-implantation bovine embryos Hum. Reprod., May 1, 2001; 16(5): 893 - 901. [Abstract] [Full Text] [PDF]
|
|