Human Reproduction, Vol. 15, No. 7, 1469-1475,
July 2000
© 2000 European Society of Human Reproduction and Embryology
An immunohistochemical analysis of fibroid vasculature
Department of Obstetrics & Gynaecology, Monash University, Melbourne, Victoria 3168, Australia
This study aimed to compare vascular parameters between fibroid and myometrium. From 10 uteri, specimens were taken from small fibroids (<0.5 cm), from the inner and outer parts of large fibroids (>3 cm), and from myometrium. Antibodies to endothelial cell markers CD31, CD34, factor VIII-related antigen (FVIII), and Ulex europaeus lectin were used in routine immuno- and lectin chemistry protocols. Parameters calculated were vascular area (VA), microvascular density (MD) and vascular luminal diameter. VA measures showed that myometrium had a greater area stained than small fibroids (P = 0.03) using CD31 and both inner (P = 0.04) and outer (P = 0.01) regions of large fibroids using FVIII, and than all groups (small, P = 0.02; inner, P = 0.02; outer, P = 0.006) using the lectin U. europaeus. MD was higher in myometrium than all uterine fibroid groups (small, P = 0.009; inner, P = 0.01; outer, P = 0.01) using U. europaeus lectin, than both regions of large (inner, P = 0.04; outer, P = 0.02) fibroids using FVIII, and than outer regions of large fibroids using CD31 (P < 0.05). There were significantly larger diameter vessels in myometrium and large fibroids compared with small fibroids using CD34, FVIII and the lectin U. europaeus (P 
0.04). These differences in vasculature may represent differences in angiogenesis and vascular remodelling.
Key words: fibroids/immunohistochemistry/leiomyoma/myometrium/vasculature
1 To whom correspondence should be addressed at: Department of Obstetrics & Gynaecology, Monash University, Melbourne,Victoria 3168, Australia. E-mail: beverley.vollenhoven{at}med.monash.edu.au
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