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Human Reproduction, Vol. 15, No. 7, 1484-1489, July 2000
© 2000 European Society of Human Reproduction and Embryology

Chlamydia trachomatis reactive T lymphocytes from upper genital tract tissue specimens

A. Kinnunen1, P. Molander3, A. Laurila1, I. Rantala4, R. Morrison5, M. Lehtinen2, R. Karttunen6, A. Tiitinen3, J. Paavonen3 and H.-M. Surcel1,7

1 National Public Health Institute, Aapistie 1, Box 310, 90101 Oulu, Finland and 2 Helsinki, 3 Department of Obstetrics and Gynaecology, University of Helsinki, Helsinki, 4 Pathology Unit, Tampere University Hospital, Tampere, 5 Department of Medical Microbiology, University of Oulu, Finland and 6 Department of Microbiology, Montana State University, Bozeman, MT, USA

Chlamydia trachomatis infection is associated with pelvic inflammatory disease (PID) and tubal factor infertility (TFI). We investigated the role of C. trachomatis as a target antigen of endometrial and salpingeal tissue lymphocytes derived from PID and TFI patients. Antigen specificity of the tissue originated T lymphocyte lines (TLL) was tested against C. trachomatis elementary bodies and chlamydial heat shock protein 60 (CHSP60). C. trachomatis antigen stimulated proliferation in two out of eight endometrial TLL derived from PID patients and three out of four TLL derived from TFI patients. All (n = 4) TLL derived from the salpingeal specimens responded to CHSP60 compared with only one out of 12 TLL derived from the endometrial specimens. In-vivo expression of interferon-gamma (IFN-{gamma}) mRNA revealed that it was present in nine of 13 specimens obtained from PID patients. The dominant activity of type-1 T lymphocytes was confirmed by the in-vitro production of IFN-{gamma} (median 1007 pg/ml) from all (n = 5) C. trachomatis specific TLL while IL-5 secretion was lower (median 779 pg/ml). In conclusion, C. trachomatis reactive TLL were established from in-vivo activated lymphocytes from the upper genital tract tissue of PID and TFI patients. The reactivity of the salpingeal TLL to CHSP60 provided further evidence that immunoreactivity to CHSP60 is a predominant response in patients with tubal damage.

Key words: cell-mediated immunity/heat shock protein 60/IFN-{gamma}/pelvic inflammatory disease/tubal factor infertility

7 To whom correspondence should be addressed at: National Public Health Institute, Aapistie 1, Box 310, 90101 Oulu, Finland. E-mail: helja_marja.surcel{at}ktl.fi


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A.H. Kinnunen, H-M. Surcel, M. Lehtinen, J. Karhukorpi, A. Tiitinen, M. Halttunen, A. Bloigu, R.P. Morrison, R. Karttunen, and J. Paavonen
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