Human Reproduction, Vol. 15, No. 7, 1592-1596,
July 2000
© 2000 European Society of Human Reproduction and Embryology
Embryonic development in vitro is compromised by the ICSI procedure
Reproductive Medicine, BioScience Centre, International Centre for Life, Newcastle upon Tyne NE1 4EP, UK
The implantation rates achieved with intracytoplasmic sperm injection (ICSI) are equivalent to those with conventional in-vitro fertilization (IVF) but information on embryonic development in vitro after ICSI is scant. In this paper we compare blastocyst formation after IVF and ICSI; we have also investigated the effect of the ICSI procedure with internal control of extrinsic (including paternal) factors. The first series comprised cases of IVF treatment (n = 101) for tubal infertility and ICSI (n = 96) for male infertility. The proportions of embryos developing to the blastocyst stage was significantly lower after ICSI (8.9%, P < 0.001) than after conventional IVF (23.5%). In order to investigate the effect of the ICSI procedure in isolation, blastocyst formation was analysed in a second series of eight cases, in which sibling oocytes were non-selectively subjected to ICSI (n = 78) or IVF (n = 67) with spermatozoa from the same semen sample. It was found that 20% of ICSI embryos and 50% of IVF embryos formed blastocysts (P < 0.01), demonstrating that the ICSI procedure contributes to a reduced capacity for blastocyst formation in vitro.
Key words: blastocyst formation/ICSI/IVF/oocyte/spermatozoa
1 To whom correspondence should be addressed.E-mail: Mary.Herbert{at}ncl.ac.uk
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