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Human Reproduction, Vol. 15, No. 8, 1667-1671, August 2000
© 2000 European Society of Human Reproduction and Embryology

Fourier transform infrared spectroscopy of follicular fluids from large and small antral follicles

Non Thomas1, Royston Goodacre2, Éadaoin M. Timmins2, Marco Gaudoin1 and Richard Fleming1,3

1 3Assisted Conception Service, Glasgow Royal Infirmary University NHS Trust, Glasgow, G31 2ER and 2 Institute of Biological Sciences, Cledwyn Building, University of Wales, Aberystwyth, Ceredigion, Wales, SY23 3DD, UK

Fourier transform infrared spectroscopy (FTIR) was used to obtain `biochemical fingerprints' for the constitution of follicular fluids from large and small antral luteinized follicles (n = 54 pairs). All samples gave reproducible characteristic biological infrared absorption spectra, with recognizable amide I protein vibrations and acyl vibrations from fatty acids. Discriminant function analysis of the first derivative FTIR spectra, together with hierarchical cluster analysis used to construct a dendrogram, showed fluid from large follicles formed a homogeneous closely related cluster, whilst that from small follicles was distinct from the large, and heterogeneous in nature. The large follicle fluids showed closer biochemical similarity to each other than to the corresponding fluid taken from small matched follicles. An artificial neural network was trained and following validation with an independent test set, successfully distinguished follicular fluids from large and small follicles. The sex steroid concentrations in the fluids from large and small follicles were significantly different. These results show that fluid from large follicles is distinct in biochemical nature from that from small follicles, but the degree of homogeneity implies size-specific changes take place. These may have consequences for the developmental potential of the oocyte.

Key words: artificial neural networks/follicle fluid/follicle size/fourier transform infrared spectroscopy/steroid analysis

3 To whom correspondence should be addressed at: Assisted Conception Service, Glasgow Royal Infirmary University NHS Trust, Glasgow G31 2ER, UK. E-mail: gqta13{at}udcf.gla.ac.uk


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