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Human Reproduction, Vol. 15, No. 8, 1717-1722, August 2000
© 2000 European Society of Human Reproduction and Embryology

Sperm chromatin structure assay parameters as predictors of failed pregnancy following assisted reproductive techniques

K.L. Larson1,4, C.J. DeJonge2,3, A.M. Barnes3, L.K. Jost1 and D.P. Evenson1

1 Department of Chemistry and Biochemistry, South Dakota State University, Brookings, SD 57007, 2 Department of Obstetrics and Gynecology, University of Minnesota, Minneapolis, MN 55454 and 3 Department of Obstetrics and Gynecology, University of Nebraska Medical Center (UNMC), Omaha, NE, USA

The predictive value of sperm chromatin integrity for pregnancy outcome following in-vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI) was studied in 24 men attending a university-based assisted reproductive techniques laboratory using the flow cytometric sperm chromatin structure assay (SCSA). The SCSA is a measure of the susceptibility of sperm DNA to low pH-induced denaturation in situ. The mean percentage of spermatozoa in the neat sample demonstrating DNA denaturation was significantly lower in the seven men that initiated a pregnancy (15.4 ± 4.6, P = 0.01) than in the 14 men who did not initiate a pregnancy (31.1 ± 3.2). No pregnancies resulted if >=27% of the spermatozoa in the neat semen sample showed DNA denaturation. These data demonstrate that SCSA parameters are independent of conventional semen parameters. Furthermore, the SCSA may allow physicians to identify male patients for whom IVF and ICSI will be unlikely to result in pregnancy initiation.

Key words: chromatin structure/human fertility/in-vitro fertiliza- tion/intracytoplasmic sperm injection/SCSA

4 To whom correspondence should be addressed at: Department of Chemistry and Biochemistry, Box 2170, South Dakota State University, Brookings, SD 57007, USA.E-mail: kjerstilea{at}hotmail.com


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