Human Reproduction, Vol. 15, No. 9, 1947-1952,
September 2000
© 2000 European Society of Human Reproduction and Embryology
Day 5 versus day 3 embryo transfer: a controlled randomized trial
1 Departments of Pathology and Laboratory Medicine and 2 Obstetrics and Gynecology, King Faisal Specialist Hospital and Research Center, P.O.Box 3354, Riyadh, Saudi Arabia
Blastocyst transfer has been suggested to improve implantation rate without affecting pregnancy rate. The aim of this study was to compare the pregnancy and implantation rates of day 3 and 5 transfers in a prospective randomized manner. Patients with four or more zygotes were randomly allocated on day 1 to either day 3 or 5 transfers. Fertilization was achieved through regular IVF or intracytoplasmic sperm injection. Zygotes were kept in Medicult IVF medium for day 3 transfers and transferred into G1.2 and G2.2 on day 1 and 3 respectively for day 5 transfers. The morphologically best two or three embryos or blastocysts were chosen for transfer in both groups. Overall pregnancy rates per embryo transfer were the same (39%) in day 3 and 5 transfers. Implantation rates were 21 and 24% for day 3 and 5 transfers respectively. The pregnancy and implantation rates for day 5 transfers were significantly affected by the availability of at least one blastocyst to transfer and the number of zygotes. The number of good quality embryos on day 3 also significantly affected pregnancy and implantation rates on day 5 transfers. Multiple gestation rate, number of abortions and ongoing pregnancies were similar in both groups. In conclusion, day 3 and 5 transfer had similar pregnancy, implantation and twinning rates. Currently, day 5 transfers have no advantages over day 3 transfers.
Key words: blastocyst/embryo transfer/sequential media
3 To whom correspondence should be addressed at: King Faisal Specialist Hospital and Research Center, P.O.Box 3354 MBC#52, Riyadh, Saudi Arabia. E-mail: jaroudi{at}kfshrc.edu.sa
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