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Human Reproduction, Vol. 16, No. 10, 2206-2210, October 2001
© 2001 European Society of Human Reproduction and Embryology

Combined screening for Chlamydia trachomatis and squamous intra-epithelial lesions using a single liquid-based cervical sample

J.L. Anguenot1,5, F. de Marval2, P. Vassilakos3, R. Auckenthaler4, V. Ibéchéole1 and A. Campana1

1 Department of Gynaecology and Obstetrics, University Hospitals of Geneva, 2 Department of Molecular Biology, Institut Bio-Analytique, and Centre for Cytology and Clinical Pathology (Cytopath), 3 Institute of Pathology and 4 Bacteriology Central Laboratory, University Hospitals of Geneva, Geneva, Switzerland

BACKGROUND: Cervicitis is believed to alter cytological interpretation and could compromise a combined screening for Chlamydia trachomatis (CT) cervicitis and squamous intra-epithelial lesions (SIL). Liquid-based cytological methods have been shown to limit obscuring factors and permit the detection of infectious agents by DNA amplification techniques. The aim of this study was to evaluate a combined screening for SIL and CT cervicitis with a single liquid-based cervical sample. METHODS: Two cervical samples were obtained from each of 590 women considered at high risk for CT. The modified Ligase chain reaction (LCR) procedure for CT detection using specimens collected in the AutoCyte's preservative fluid was compared with the conventional Abbott LCx method using cervical swabs. We have also compared the percentage of inflammatory specimens and adequacy of cellular material in the populations of CT+ and CT- women. RESULTS: The results show total agreement for 588 of 590 cervical samples using the two LCR protocols (Kappa = 0.96; 95% confidence interval: 0.91–1.00). The quality of cervical cytology was not compromised by CT cervicitis. CONCLUSIONS: We demonstrated the feasibility of combined screening for CT and SIL with a single liquid-based cervical sample.

Key words: cervical screening/Chlamydia trachomatis/DNA amplification techniques/liquid-based cervical sample/squamous intra-epithelial lesion

5 To whom correspondence should be addressed at: Department of Gynaecology and Obstetrics, University Hospitals of Geneva, 1211 Geneva 14, Switzerland. E-mail: Jean-Luc.Anguenot{at}hcuge.ch


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