Human Reproduction, Vol. 16, No. 12, 2628-2631,
December 2001
© 2001 European Society of Human Reproduction and Embryology
Laser assisted immobilization of spermatozoa prior to intracytoplasmic sperm injection in humans
Women's General Hospital, IVF-Unit, A-4020 Linz, Austria
BACKGROUND: The conventional method of immobilization of spermatozoa prior to intracytoplasmic sperm injection (ICSI) is mechanical breakage of the tail by pressing it against the bottom of the injection dish. METHODS: This prospective self-controlled study was set up to evaluate the potential of a non-contact 1.48 µm wavelength diode laser in terms of immobilization. In addition, the fertilization rate and further development potential of such zygotes were investigated. The patients included in our study (n = 60) had oestradiol concentrations >2000 pg/ml, and thus a relatively high number of MII oocytes could be expected. Approximately half the oocytes were injected with laser treated spermatozoa (n = 262, study group) and the other half with mechanically immobilized spermatozoa (n = 252, control group). RESULTS: No significant differences between the two groups in terms of fertilization rate, early cleavage or blastocyst formation were observed. However, time required for identification, aspiration and injection of a potential spermatozoa was significantly shorter in the laser immobilized sperm group (P < 0.001). CONCLUSIONS: The application of a non-contact diode laser for sperm immobilization prior to ICSI is a potentially useful alternative to the conventional mechanical approach.
Key words: blastocyst formation/early cleavage/fertilization rate/immobilization of spermatozoa/non-contact laser
1 To whom correspondence should be addressed. E-mail: Thomas.Ebner{at}li.lkh.ooe.gv.at
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