Human Reproduction, Vol. 16, No. 12, 2658-2661,
December 2001
© 2001 European Society of Human Reproduction and Embryology
Early cleavage of human embryos: an effective method for predicting successful IVF/ICSI outcome
Fundaç
o Universitária de Endocrinologia e Fertilidade (FUEFE), Rua Alcides Cruz 101, CEP: 90.630–160, Porto Alegre, RS, Brazil
BACKGROUND: The need for effective parameters for selecting the best embryos is paramount when a large number of them are available for transfer. Other studies have reported that transfer of pre-selected embryos, based on cleavage to the 2-cell stage at 25 h and 27 h post-insemination/intracytoplasmic sperm injection (ICSI), increases implantation and pregnancy rates. We investigated whether extending the time for selection of cleaved embryos to 29 h post-insemination/ICSI had a similar effect on pregnancy and implantation rates. METHODS: Cleavage to the 2-cell stage was assessed at 25, 27 and 29 h post-insemination/ICSI. Embryos that had cleaved at any of these time points were designated as `early cleavage' (EC), while others were designated as `non-early cleavage' (NEC). EC embryos were selected and preferentially transferred. RESULTS: EC occurred in 57% of the cycles (61% IVF; 51% ICSI). Significantly (P = 0.02) more clinical pregnancies occurred in the EC group (23/42, 55%) compared with the group that had no embryo undergoing first cleavage up to 29 h post-insemination/ICSI (8/32, 25%). The EC group of patients was significantly younger than the NEC. CONCLUSION: Transfer of selected embryos that reached the 2-cell stage between 25 and 29 h post-insemination/ICSI is a reliable prognostic tool for patients undergoing assisted reproduction techniques.
Key words: assessment times/early cleavage/embryo selection/pregnancy rates
1 To whom correspondence should be addressed. E-mail: adriana{at}orion.ufrgs.br
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