Human Reproduction, Vol. 16, No. 2, 306-312,
February 2001
© 2001 European Society of Human Reproduction and Embryology
Embryo development and chromosomal anomalies after ICSI: effect of the injection procedure*
Research Institute of Growth and Development (GROW), University of Maastricht, 1 Department of Obstetrics and Gynaecology and 2 Department of Molecular Cell Biology and Genetics, Academic Hospital Maastricht, Maastricht, The Netherlands
Intracytoplasmic sperm injection (ICSI) is a delicate procedure requiring considerable skills of the person performing it. Theoretically, the injection procedure could damage cytoplasmic structures in the oocyte, resulting in sublethal cellular injury and/or numerical chromosomal abnormalities that could lead to impaired embryonic development. In the present study, features of the injection procedure were evaluated in a total of 2924 oocytes from 305 cycles. Development to the blastocyst stage was found to be compromised in a group of surplus embryos originating from oocytes in which >6 pl of cytoplasm was aspirated into the injection pipette during the ICSI procedure. Characteristics of the injection procedure as well as blastocyst development of surplus embryos was shown to be significantly different between the four technicians performing the ICSI. Neither the volume of cytoplasm aspirated during the injection procedure, nor the position of the polar body (6 o'clock or 12 o'clock) influenced the mean incidence of disomic cells per blastocyst as revealed by fluorescence in-situ hybridization using probes specific for chromosomes X, Y and 18. In conclusion, certain technical aspects of the injection procedure can affect subsequent embryonic development to the blastocyst stage, but do not seem to influence the rate of chromosomal abnormalities that occur in human pre-implantation embryos.
Key words: chromosomal abnormalities/fluorescence in-situ hybridization/human blastocysts/ICSI
3 To whom correspondence should be addressed at: IVF-Laboratory, Department of Obstetrics and Gynaecology, Academic Hospital Maastricht, P.O. Box 5800, 6202 AZ, Maastricht, The Netherlands
* These data were presented in part at the 15th Annual Meeting of the European Society for Human Reproduction and Embryology (ESHRE), Tours, France, June 27–30, 1999
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