Human Reproduction, Vol. 16, No. 5, 1037-1045,
May 2001
© 2001 European Society of Human Reproduction and Embryology
Oestrogenic potencies of Zeranol, oestradiol, diethylstilboestrol, Bisphenol-A and genistein: implications for exposure assessment of potential endocrine disrupters
Department of Growth and Reproduction, Rigshospitalet, Copenhagen, Denmark
We have compared the oestrogenic potency of the synthetic oestrogen Zeranol, used as a growth promoter in meat production, and five related compounds, with the potency of 17ß-oestradiol, diethylstilboestrol (DES), genistein, and Bisphenol-A. The potency was assayed by analysing differences in expression levels of endogenous oestrogen-regulated genes in human MCF7 cells, treated with different concentrations of the compounds. Zeranol, 17ß-oestradiol and DES were about equally potent, genistein was four to six orders of magnitude less potent than 17ß-oestradiol but an order of magnitude more potent than Bisphenol-A. There were gene specific differences, the PS2 and TGFß3 genes were about equally sensitive to Zeranol, 17ß-oestradiol and DES whereas a down-regulation of MRG1/p35srj could be detected at fmol/l concentrations of Zeranol whereas 17ß-oestradiol was several orders of magnitude less potent. GST mu3 was sensitive to fmol/l concentrations of 17ß-oestradiol but much less sensitive to Zeranol and DES. The very high potency of Zeranol compared with other potential endocrine disrupters suggests that Zeranol intake from beef products could have greater impact on consumers than the amounts of the known or suspected endocrine disrupters that have been found in food. Since little data is available in man, there is an urgent need for reliable measurements of the concentration of Zeranol in human serum after ingestion of meat products from treated animals.
Key words: ATB0+/GST mu3/p35srj/PS2/Zeranol
1 To whom correspondence should be addressed at: Department of Growth and Reproduction, Section GR-5064, Rigshospitalet, Blegdamsvej 9, DK-2100 Copenhagen, Denmark. E-mail: henrik.leffers{at}biobase.dk
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